Acta Ortopédica Mexicana

Álvarez E, Ripalda P, Forriol F
Cryopreservation of cartilaginous grafts
Acta Ortop Mex 2003; 17 (3)

Language: Español
References: 37
Page: 157-159
PDF: 36.54 Kb.

[Fulltext - PDF]

ABSTRACT

The autografts have had good results. However, they have some availability problems. In order to avoid this difficulties, techniques like the cryopreservation, had been developed. This technique permits an unlimited time of storage by stopping the biological time of a tissue. Nevertheless, the cryopreservation of cartilaginous grafts, in comparison to other musculoskeletal grafts, represent a problem, and the actual techniques are not simple methods and do not assure the integrity of the tissue completely, because the process can damage the cells, the extracellular matrix, or both, which is known as cryoinduced damage. In the modern cryopreservation protocols the cryoprotector agents have an important function, however, is also well known their cytotoxic effect. The develop of an effective cryopreservation method is an essential requirement to obtain good results in the cartilaginous and osteocartilaginous transplants, which will be directly reflected in the capacity to maintain an acceptable cellular viability.


Key words: cartilage, articular, cryopreservation, allograft.