Acta Ortopédica Mexicana

Álvarez E, Ripalda P, Forriol F
Cryopreservation of cartilaginous grafts
Acta Ortop Mex 2003; 17 (3)

Language: Español
References: 37
Page: 157-159
PDF: 36.54 Kb.

[Fulltext - PDF]


The autografts have had good results. However, they have some availability problems. In order to avoid this difficulties, techniques like the cryopreservation, had been developed. This technique permits an unlimited time of storage by stopping the biological time of a tissue. Nevertheless, the cryopreservation of cartilaginous grafts, in comparison to other musculoskeletal grafts, represent a problem, and the actual techniques are not simple methods and do not assure the integrity of the tissue completely, because the process can damage the cells, the extracellular matrix, or both, which is known as cryoinduced damage. In the modern cryopreservation protocols the cryoprotector agents have an important function, however, is also well known their cytotoxic effect. The develop of an effective cryopreservation method is an essential requirement to obtain good results in the cartilaginous and osteocartilaginous transplants, which will be directly reflected in the capacity to maintain an acceptable cellular viability.

Key words: cartilage, articular, cryopreservation, allograft.


  1. Baker H, Schork P: The effects of glycerol and ethylene glycol on the excitability and contractility of frog ventricular muscle. Cryobiology 1972; 9: 321-22.

  2. Blaser RB, Matthews LS: Complications of prosthetic knee arthroplasty. In: Complications in orthopedic surgery. CH Epps (ed), Philadelphia, 1994: 1057-86.

  3. Brown JB: Preserved and fresh homotransplants of cartilage. Surg Gynecol Obstet 1940; 70: 1079-82.

  4. Burnstein D, Gray ML, Hartman AL, Gipe R, Foy BD: Diffusion of small solutes in cartilage as measured by NMR spectroscopy and imaging. J Orthop Res 1993; 11: 465-78.

  5. Curran RC, Gibson T: The uptake of labelled sulphate by human cartilage cells and its use as a test for viability. Proc R Soc London B 1956; 144: 562-76.

  6. Fahy GM, Khirabadi BS, Mehl P: Equipment, solutions, perfusion techniques and medications permitting survival of kidneys perfused with vitrificable media. Cryobiol 1991; 28: 511-12.

  7. Fahy GM: The potential future utility of organ cryopreservation. Cryobiol 1991; 28: 513-15.

  8. Flynn JM, Springfield DS, Mankin HJ: Osteoarticular allografts to treat distal femoral osteonecrosis. Clin Orthop 1994; 303: 38-43.

  9. Gibson T: Viability of cartilage after freezing. Proc R Soc London B 1957; 147: 528-29.

  10. Hurting M, Mcgann LE, Mcpherson R, Muldrew K, Novak K, Scharchar NS: Surgical and analytical techniques for assessment of transplanted articular cartilage: A pilot study. Cryobiol 1992; 29: 45-56.

  11. Karow AM: Cryopreservation: Pharmacological considerations. In: Organ preservation for transplantation. Karow AM Jr., Abouna GJM, Humphries AL (eds). Boston, Little Brown, 1974: 86-107.

  12. Karow AM: Dimethylsulfoxide effect on Myocardial-Adenoreceptors (letter). J Pharm and Pharmacol 1972; 24: 419-21.

  13. Khirabadi BS, Fahy GM, Antonovich T, Sabnis S, Saur J: Life support function of rabbit kidneys cooled to -30°C. Cryobiol 1992; 29: 721-22.

  14. Loretz LJ, Li AP, Flye MW, Wilson AG: Optimization of cryopreservation procedures for rat and human hepatocytes. Xenobiotica 1989; 19: 489-98.

  15. Mazur P: Causes of injury in Frozen and Thawed cells. Fed Proc 1965; 24: 175-82.

  16. Mazur P: Cryobiology. The Freezing of biological systems. Science 1970; 168: 939-49.

  17. McDermott AGP, Langer F, Pritzker KPH, Gross AE: Fresh small-fragment osteochondral allografts. Long-Term follow-up study on first 100 cases. Clin Orthop 1985; 197: 96-102.

  18. McGann LE, Muldrew K, Hurtig M, Novak K, Scharchar NS: Cryopreservation of articular cartilage for transplantation (Abstract). Cryobiol 1993; 30: 659.

  19. McGann LE, Stevenson M, Mcallister D, Muldrew K, Shachar NS: Kinetics of osmotic water movement in chondrocytes isolated from articular cartilage and applications to cryopreservation. J Orthop Res 1988; 6: 106-15.

  20. Meryman HT, Williams RJ, Douglas M: Freezing injury from “solution effects” and its prevention by natural or artificial cryoprotection. Cryobiology 1971; 14: 287-302.

  21. Meryman HT: Freezing injury and its prevention in living cells. Ann Rev Biophys and Bioeng 1974; 3: 341-63.

  22. Messner K, Msletius W: The long -term prognosis for severe damage to weight bearing cartilage in the knee. Acta Orthop Scand 1996; 67: 165-68.

  23. Meyers MH, Akeson W, Convery FR: Resurfacing of the knee with fresh osteochondral allograft. J Bone Joint Surg 1989; 71A: 704-13.

  24. Muldrew K, Mcgann LE: The osmotic rupture hypothesis of intracellular freezing injury. Biophys J 1994; 66: 532-41.

  25. O’Connor GB, Pricce GW: Refrigerated cartilage isografts. Surg Gynecol Obstet 1938; 67: 796-797.

  26. Oakeshott RD, Farine Y, Pritzker KPH, Langer F, Gross AE: A clinical and histological analysis of failed fresh osteochondral allografts. Clin Orthop 1988; 233: 283-94.

  27. Okouchi Y, Tamaki T, Kozaki M: The optimal temperature for hypothermic liver preservation in the rat. Transplantation 1993; 54: 1129-30.

  28. Peer LA: Experiments on alcohol preserved cartilage in humans. Arch Otolaryngol 1938; 27: 42-4.

  29. Peer LA: Fate of livineg and dead cartilage transplanted in humans. Surg Gynecol Obstet 1939; 68: 603-4.

  30. Prudden TM: Experimental studies on the transplantation of cartilage. Am J Med Sci 1881; 82: 360-61.

  31. Reddi AH: Extracellular bone matrix dependent local induction of cartilage and bone. J Rheumatol 1983; 10(Suppl. 11): 67-78.

  32. Salter RB: The biologic concept of continuous passive motion of synovial joints: the first 18 years of research and its clinical application. Clin Orthop 1989; 242: 12-8.

  33. Smith AU: Survival of frozen chondrocytes isolated from cartilage of adult mammals. Nature 1965; 205: 782-4.

  34. Tomford WW, Duff GP, Mankin HJ: Experimental freeze preservation of chondrocytes. Clin Orthop 1985; 197: 11-4.

  35. Tomford WW: Current concepts review: Transmission of diseases through transplantation of musculoskeletal allografts. J Bone Joint Surg 1995; 77A: 1742-54.

  36. Wakitani S, Goto T, Pineda SJ, Young RG, Mansour J, Goldberg VM, Caplan AI: Mesenchymal cell-based repair of large full-thickness defects of articular cartilage. J Bone Joint Surg 1994; 76A: 579-92.

  37. Weiner ND, Lu MY, Rosoff M: Interactions of dimethyl sulfoxide with lipid and protein monolayers. J Pharmaceut Sci 1972; 61: 1098-101.