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Revista de Divulgación del INSTITUTO DE MEDICINA FORENSE de la UNIVERSIDAD VERACRUZANA

2021, Número 2

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Rev Mex Med Forense 2021; 6 (2)


Eficiencia de cinco marcadores X-STR para identificación forense en población mestiza del Occidente de México

Medina-Mora Y, Javalera-Castro D, Rivas F, Valle Y, Del Toro-Arreola A, Daneri-Navarro A, Topete A, Padilla-Gutiérrez JR, Quintero-Ramos A

Idioma: Español
Referencias bibliográficas: 44
Paginas: 38-58
Archivo PDF: 438.96 Kb.


RESUMEN

Introducción: Las repeticiones cortas en tándem del cromosoma X (X-STR) son utilizadas en la identificación forense y pruebas de parentesco biológico. Actualmente existen varios estudios en los X-STR, sin embargo, el panel incluye varios marcadores del mismo grupo de ligamiento. Al incluir marcadores de diferente grupo de ligamiento la prueba es más eficiente. Objetivo: Establecer un panel de cinco loci X-STR para su aplicación en estudios genéticos forenses en población mestiza del Occidente de México. Metodología: Se tipificaron cinco loci X-STR en 379 muestras de individuos del Occidente de México mediante PCR, geles de poliacrilamida al 7% y tinción con plata. Se estimaron las frecuencias alélicas y haplotípicas, así como los parámetros forenses. Resultados: Los datos combinados mostraron un poder de discriminación de 0.999389 y 0.999995 en hombres y mujeres respectivamente. La combinación de la probabilidad media de exclusión fue 0.9998797 para casos de tríos y 0.990701 para dúos. Discusión: Diversos reportes, que utilizan el sistema Decaplex, obtienen valores de discriminación en mujeres de 99.9999% y en hombres de 99.9998%, resultados muy cercanos al nuestro y con sólo utilizar cinco marcadores. Conclusión: los cinco loci X-STR analizados son altamente informativos para uso en pruebas forenses.


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