Sasmakov SA, Ashirov ON, Abdurakhmanov JM, Khasanov SSh, Eshboev FB, Yusupova EG, Piyakina GA, Azimova SS

Idioma: Ingles.
Referencias bibliográficas: 21
Paginas: 27-32
Archivo PDF: 549.67 Kb.

RESUMEN

El objetivo de este trabajo es la expresión de la región PreS2-S del antígeno de superficie del virus de la hepatitis B en la levadura Pichia pastoris. Se clonó un fragmento de ADNc que codifica la proteína PreS2-S del VHB en vectores de transferencia de levadura. A partir de los nuevos plásmidos clonados pPIC3.5-PreS2-S (8707 pb) y pPIC9-PreS2-S (8980 pb) se obtuvieron las cepas recombinantes de P. pastoris productoras de la región PreS2-S del antígeno de superficie del VHB. La electroforesis PAGE y la inmunotransferencia de la proteína PreS2-S recombinante obtenida confirman el peso molecular (34 kDa) y la alta especificidad a los anticuerpos contra el VHB.

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