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Revista Latinoamericana de Microbiología

2006, Número 2

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Microbiología 2006; 48 (2)


Evaluación in silico de un novedoso chip de DNA basado en tecnología de huella genómica para identificación viral

Méndez TA, Flores CP, Guerra TA, Jaimes DH, Reyes RE, Maldonado RA, Espinosa LM, Maldonado RR, Beattie KL

Idioma: Ingles.
Referencias bibliográficas: 40
Paginas: 56-65
Archivo PDF: 182.67 Kb.


RESUMEN

En este trabajo se evaluó, “in silico”, la identificación de organismos por medio de su huella genómica. Las secuencias genómicas de 94 HPVs se sometieron a hibridación virtual sobre un chip de DNA que contiene 342 sondas. Este Sensor Universal de Huella Genómica o UFC está integrado por un conjunto de sondas representativo de todas las secuencias posibles de 8 nucleótidos de longitud que contienen al menos dos diferencias, internas y espaciadas, entre todas ellas. El análisis de hibridación virtual permitió calcular las huellas genómicas que representan las señales producidas por la hibridación de las sondas permitiendo a lo mucho una base no apareada. Las huellas genómicas fueron comparadas entre sí para obtener mediciones de distancias entre todos los pares posibles. Se utilizó una técnica de extensión del alineamiento para considerar solo las señales compartidas por dos genomas correspondientes a la hibridación de las sondas contra sitios homólogos. Se construyó un árbol filogenético a partir de las distancias entre las huellas genómicas utilizando el algoritmo Neighbor-Joining del programa Phylip 3.61. Este árbol fue comparado con el obtenido a partir del alineamiento de los genomas completos de HPV obtenido con el programa Clustal_X 1.83. La similitud entre los árboles obtenidos por ambos métodos sugiere que el UFC-8 es capaz de discriminar con precisión los genomas virales. El análisis comparativo de las huellas genómicas indica que el UFC-8 es capaz de distinguir los tipos y subtipos de HPV.


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