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>Revistas >Revista Latinoamericana de Microbiología >Año 2006, No. 3-4


Juárez-Verdayes MA, Reyes-López MA, Cancino-Díaz ME, Muñoz-Salas S, Rodríguez-Martínez S, Zavala-Díaz SFJ, Hernández-Rodríguez CH, Cancino-Díaz JC
Isolation, vancomycin resistance and biofilm production of Staphylococcus epidermidis from patients with conjunctivitis, corneal ulcers, and endophthalmitis
Microbiología 2006; 48 (3-4)

Idioma: Inglés
Referencias bibliográficas: 59
Paginas: 238-246
Archivo PDF: 115.83 Kb.


Texto completo




RESUMEN

The infection frequency associated to bacterial conjunctivitis, corneal ulcers (CU), and endophthalmitis was studied along a five years period. The isolation and identification of microorganisms were performed by culture-based methods and biochemical test respectively. Also, a nested PCR to detect gram-negative and gram-positive bacteria in the clinical samples was assayed. Nested PCR was a more efficient method than culture to detect bacteria in the samples. The most frequently isolated species was Staphylococcus epidermidis, a bacterium commonly considered as a human saprophyte. The S. epidermidis strains from conjunctivitis, CU, and endophthalmitis exhibited 46, 33.9, and 34.1% of oxacilin-resistance respectively. A total of 28% of intermediate-vancomycin resistance (MIC = 8-16 μg/ml) was observed among S. epidermidis strain collection. The UPGMA cluster analysis of the multiresistance profile data of intermediate vancomycin-resistant S. epidermidis strains showed a high phenotypic diversity and no relationship between each group and their clinical origin. The biofilm formation capacity was broadly distributed (66%), particularly among intermediate-vancomycin strains (› 75%). In brief, S. epidermidis displayed a high diversity of antibiotic resistance profiles and biofilm formation capacity. These phenotypic traits could explain the high isolation frequency of S. epidermidis from ocular infections and oblige to review the saprophytic status of these bacteria.


Palabras clave: Conjunctivitis, corneal ulcers, endophthalmitis, Staphylococcus epidermidis, vancomycin-resistance.


REFERENCIAS

  1. Ahanotu EN, Stone JH, McAllister SK, Miller JM, Ahearn DG. 2001. Vancomycin resistance among strains of Staphylococcus epidermidis: effects on adherence to silicone. Curr Microbiol 43: 124-128.

  2. Ammendolia MG, Di Rosa R, Montanaro L, Arciola CR, Baldassarri L. 1999. Slime production and expression of the slime-associated antigen by staphylococcal clinical isolates. J Clin Microbiol 37:3235-3238.

  3. An YH, Friedmann RJ. 1998. Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. J Biomed Mater Res 43:338-348.

  4. Anand AR, Madhavan HN, Therese KL. 2000. Use of polymerase chain reaction (PCR) and DNA probe hybridization to determine the Gram reaction of the infecting bacterium in the intraocular fluids of patients with endophthalmitis. J Infect 41:221-226.

  5. Asaria RH, Downie JA, McLauglin-Borlace L, Morlet N, Munro P, Charteris DG. 1999. Biofilm on scleral explants with and without clinical infection. Retina 19:447-450.

  6. Aubert G, Passot S, Lucht F, Dorche G. 1990. Selection of vancomycin- and teicoplanin-resistant Staphylococcus haemolyticus during teicoplanin treatment of S. epidermidis infection. J Antimicrob Chemother 25:491-493.

  7. Benz MS, Scott IU, Flynn HW Jr, Unonius N, Miller D. 2004. Endophthalmitis isolates and antibiotic sensitivities: a 6-year review of culture-proven cases. Am J Ophthalmol 37:38- 42.

  8. Bourcier T, Thomas F, Borderie V, Chaumeil C, Laroche L. 2003. Bacterial keratitis: predisposing factors, clinical and microbiological review of 300 cases. Br J Ophthalmol 87:834-838.

  9. Carroll NM, Jaeger EE, Choudhury S, Dunlop AA, Matheson MM, Adamson P, Okhravi N, Lightman S. 2000. Detection of and discrimination between gram-positive and gram-negative bacteria in intraocular samples by using nested PCR. J Clin Microbiol 38:1753-1757.

  10. Christensen GD, Simson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachy EH. 1985. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 22:996-1006.

  11. Costerton J W, Stewart PS, Greenberg EP. 1999. Bacterial biofilms: a common cause of persistent infections. Science 284:1318-1322.

  12. Crampton S E, Gerke C, Schnell NF, Nichols WW, Gotz F. 1999. The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect Immun 67:5427-5433.

  13. Deighton M, Borland R, Capstick JA. 1996. Virulence of Staphylococcus epidermidis in a mouse model: significance of extracellular slime. Epidemiol Infect 117: 267-280.

  14. Del’ Alamo, L, Cereda RF, Tosin I, Miranda EA, Sader HS. 1999. Antimicrobial susceptibility of coagulase-negative staphylococci and characterization of isolates with reduced susceptibility to glycopeptides. Diagn. Microbiol. Infect Dis 34: 185-191.

  15. Ditlove J, Weidmann P, Bernstein M, Massry SG. 1977. Methicillin nephritis. Medicine (Baltimore) 56:483-491.

  16. Dunne WM, Qureshi H Jr, Pervez H, Nafziger DA. 2001. Staphylococcus epidermidis with intermediate resistance to vancomycin: elusive phenotype or laboratory artifact? Clin Infect Dis 33: 135-137.

  17. Fisch A, Salvanet A, Prazuck T, Forestier F, Gerbaud L, Coscas G, Lafaix C. 1991. Epidemiology of infective endophthalmitis in France. The French Collaborative Study Group on Endophthalmitis. Lancet 338:1373-1376.

  18. Friedman E, Peyman GA, May DR. 1978. Endophthalmitis caused by Propionibacterium acnes. Can J Ophthalmol 13:50-52.

  19. Froggatt JW, Johnston JL, Galetto DW, Archer GL. 1989. Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus. Antimicrob Agents Chemother 33:460-466.

  20. Garrett DO, Jochimsen E, Murfitt K, Hill B, McAllister S, Nelson P, Spera RV, Sall RK, Tenover FC, Johnston J, Zimmer B, Jarvis WR. 1999. The emergence of decreased susceptibility to vancomycin in Staphylococcus epidermidis. Infect Control Hosp Epidemiol 20: 167-170.

  21. Goldstein MH, Kowalski RP, Gordon YJ. 1999. Emerging fluoroquinolone resistance in bacterial keratitis: a 5-year review. Ophthalmology 106:1313-1318.

  22. Heilmann C, Schweitzer O, Gerke C, Vanittanakom N, Mack D, Gotz F. 1996. Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol 20:1083-1091.

  23. Hola V, Ruzicka F, Votava M. 2004. Differences in antibiotic sensitivity in biofilm-positive and biofilm-negative strains of Staphylococcus epidermidis isolated from blood cultures. Epidemiol Mikrobiol Immunol 53:66-69.

  24. Jaccard P. 1908. Nouvelles récherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223-270.

  25. Konig C, Schwank S, Blaser J. 2001. Factors compromising antibiotic activity against biofilms of Staphylococcus epidermidis. Eur J Clin Microbiol Infect Dis 20:20-26.

  26. Krcmery V Jr, Trupl J, Drgona L, Lacka J, Kukuckova E, Oravcova E. 1996. Nosocomial bacteremia due to vancomycin-resistant Staphylococcus epidermidis in four patients with cancer, neutropenia, and previous treatment with vancomycin. Eur J Clin Microbiol Infect Dis 15: 259-261.

  27. Kunimoto DY, Sharma S, Garg P, Gopinathan U, Miller D, Rao GN. 2000. Corneal ulceration in the elderly in Hyderabad, south India. Br J Ophthalmol 84:54-59.

  28. Lohmann CP, Winkler von Mohrenfels C, Gabler B, Reischl U, Kochanowski B. 2000. Polymerase chain reaction (PCR) for microbiological diagnosis in refractory infectious keratitis: a clinical study in 16 patients. Klin Monatsbl Augenheilkd 217:37-42.

  29. Luh KT, Hsueh PR, Teng LJ, Pan HJ, Chen YC, Lu JJ, Wu JJ, Ho SW. 2000. Quinupristin-dalfopristin resistance among gram-positive bacteria in Taiwan. Antimicrob Agents Chemother 44:3374-3380.

  30. Manly JF. 1997. Randomization, Bootstrap and Monte Carlo Methods in Biology. Chapman & Hall, London, United Kingdom.

  31. Marangon FB, Miller D, Muallem MS, Romano AC, Alfonso EC. 2004. Ciprofloxacin and levofloxacin resistance among methicillin-sensitive Staphylococcus aureus isolates from keratitis and conjunctivitis. Am J Ophthalmol 137: 453-458.

  32. Martin M, Turco JH, Zegans ME, Facklam RR, Sodha S, Elliott JA, Pryor JH, Beall B, Erdman DD, Baumgartner YY, Sanchez PA, Schwartzman JD, Montero J, Schuchat A, Whitney CG. 2003. An outbreak of conjunctivitis due to atypical Streptococcus pneumoniae. N Engl J Med 348: 1112-1121.

  33. Montan P, Lundstrom M, Stenevi U, Thorburn W. 2002. Endophthalmitis following cataract surgery in Sweden. The 1998 national prospective survey. Acta Ophthalmol Scand 80:258-261.

  34. Monzon M, Oteiza C, Leiva J, Amorena B. 2001. Synergy of different antibiotic combinations in biofilms of Staphylococcus epidermidis. J Antimicrob Chemother 48: 793-801.

  35. Monzon M, Oteiza C, Leiva J, Lamata M, Amorena B. 2002. Biofilm testing of Staphylococcus epidermidis clinical isolates: low performance of vancomycin in relation to other antibiotics. Diagn Microbiol Infect Dis 44:319-324.

  36. Musch DC, Sugar A, Meyer RF. 1983. Demographic and predisposing factors in corneal ulceration. Arch Ophthalmol 101:1545-1548.

  37. Nayak N, Satpathy G, Vajpayee RB, Pandey RM. 2001. A simple alternative method for rapid detection of slime produced by Staphylococcus epidermidis isolates in bacterial keratitis. Indian J Med Res 114:169-172.

  38. Okhravi N, Adamson P, Lightman S. 2000. Use of PCR in endophthalmitis. Ocul Immunol Inflamm 8:189-200.

  39. Okhravi N, Adamson P, Matheson MM, Towler HM, Lightman S. 2000. PCR-RFLP-mediated detection and speciation of bacterial species causing endophthalmitis. Invest Ophthalmol Vis Sci 41:1438-1447.

  40. Orden Martinez B, Martinez Ruiz R, Millan Perez R. 2004. Bacterial conjunctivitis: most prevalent pathogens and their antibiotic sensitivity. An Pediatr (Barc) 61: 32-36.

  41. Pfeltz RF, Wilkinson BJ. 2004. The escalating challenge of vancomycin resistance in Staphylococcus aureus. Curr Drug Targets Infect Disord 4:273-294.

  42. Pinna A, Zanetti S, Sotgiu M, Sechi LA, Fadda G, Carta F. 1999. Identification and antibiotic susceptibility of coagulase negative Staphylococci isolated in corneal/external infections. Br J Ophthalmol 83: 771-773.

  43. Rachid S, Ohlsen K, Witte W, Hacker J, Ziebuhr W. 2000. Effect of subinhibitory antibiotic concentrations on polysaccharide intercellular adhesin expression in biofilm-forming Staphylococcus epidermidis. Antimicrob Agents Chemother 44:3357-3363.

  44. Roussel TJ, Olson ER, Rice T, Meisler D, Hall G, Miller D. 1982. Chronic post-operative endophthalmitis associated with Actinomyces species. Arch Ophthalmol 109:60-62.

  45. Sanyal D, Johnson AP, George RC, Cookson BD, Williams AJ. 1991. Peritonitis due to vancomycin-resistant Staphylococcus epidermidis. Lancet 337: 54.

  46. Schabereiter-Gurtner C, Maca S, Rolleke S, Nigl K, Lukas J, Hirschl A, Lubitz W, Barisani-Asenbauer T. 2001. 16S rDNA-based identification of bacteria from conjunctival swabs by PCR and DGGE fingerprinting. Invest Ophthalmol Vis Sci 42:1164-1171.

  47. Schwalbe R, Stapleton JT, Gilligan PH. 1987. Emergence of vancomycin resistance in coagulase-negative staphylococci. N Engl J Med 316: 927.

  48. Sneath PHA, Sokal RR. 1973. Numerical taxonomy. The principle and practice of numerical classification. W. H. Freeman & Co., San Francisco, CA.

  49. Strauss RE. 1982. Statistical significance of species clusters in association analysis. Ecology 63:634-639.

  50. Tenover FC, Lancaster MV, Hill BC, Steward CD, Stocker SA, Hancock GA, O’Hara CM, McAllister SK, Clark NC, Hiramatsu K. 1998. Characterization of staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J Clin Microbiol 36: 1020-1027.

  51. Tetz MR, Klein U, Volcker HE. 1997. Staphylococcus-associated blepharokeratoconjunctivitis. Clinical findings, pathogenesis and therapy. Ophthalmologe 94:186-190.

  52. Therese KL, Anand AR, Madhavan HN. 1998. Polymerase chain reaction in the diagnosis of bacterial endophthalmitis. Br J Ophthalmol 82:1078-1082.

  53. Tuft SJ, Matheson M. 2000. In vitro antibiotic resistance in bacterial keratitis in London. Br J Ophthalmol 84:687-691.

  54. Upadhyay MP, Karmacharya PC, Koirala S, Tuladhar NR, Bryan LE, Smolin G, Whitcher JP. 1991. Epidemiologic characteristics, predisposing factors, and etiologic diagnosis of corneal ulceration in Nepal. Am J Ophthalmol 111:92-99.

  55. Veach LA, Pfaller MA, Barrett M, Koontz FP, Wenzel RP. 1990. Vancomycin resistance in Staphylococcus haemolyticus causing colonization and bloodstream infection. J Clin Microbiol 28: 2064-2068.

  56. Wahl JC, Katz HR, Abrams DA. 1991. Infectious keratitis in Baltimore. Ann Ophthalmol 23:234-237.

  57. Wong JS, Chan TK, Lee HM, Chee SP. 2000. Endogenous bacterial endophthalmitis: an east Asian experience and a reappraisal of a severe ocular affliction. Ophthalmology 107:1483-1491.

  58. Zheng Z, Stewart PS. 2002. Penetration of rifampin through Staphylococcus epidermidis biofilms. Antimicrob Agents Chemother 46:900-903.

  59. Ziebuhr W, Heilmann C, Gotz F, Meyer P, Wilms K, Straube E, Hacker J. 1997. Detection of the intercellular adhesin gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates. Infect Immun 65:890-896.



>Revistas >Revista Latinoamericana de Microbiología >Año2006, No. 3-4
 

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