Poznyak T, García A, Kiseleva E

Idioma: Ingles.
Referencias bibliográficas: 37
Paginas: 15-23
Archivo PDF: 201.20 Kb.

RESUMEN

Es evidente la influencia del estrés oxidativo en ciertos estados patológicos, especialmente en enfermedades crónicas. La medición de los niveles de lipoperoxidación, así como de sus subproductos, son usados de forma rutinaria como índices de estrés oxidativo; sin embargo, hasta ahora los métodos analíticos empleados en la determinación de la lipoperoxidación presentan limitaciones asociadas a la sensibilidad, especificidad y tiempo total de análisis. En la presente breve reseña, se describe el método del índice de dobles enlaces (DB-index), un método prometedor para la evaluación de trastornos en el metabolismo de los lípidos y la medición de la lipoperoxidación. El cálculo del DB-index se basa en el analizador de insaturación total (TUA patentado), cuya instrumentación se fundamenta en la propiedad de los compuestos orgánicos insaturados de integrar rápidamente ozono en el sitio de la doble ligadura (una molécula de ozono por cada doble ligadura). Mediante esta técnica, es posible determinar en un corto tiempo (1-3 minutos) y con alta precisión (± 1%) la insaturación total de los lípidos en plasma y en membranas celulares. Se discuten algunos casos notables donde el DB-index se ha establecido como criterio de diagnóstico y pronóstico en diferentes enfermedades, así como posibles aplicaciones presentes y futuras en el análisis clínico.

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