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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2014, Número S1

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Rev Invest Clin 2014; 66 (S1)


Estudio preliminar del desarrollo in vitro de un neotejido aplicando estimulación mecánica con un biorreactor como alternativa para la reconstrucción de ligamento

Bourdón-Santoyo M, Quiñones-Uriostegui I, Martínez-López V, Sánchez-Arévalo F, Alessi-Montero A, Velasquillo C, Ibarra-Ponce de León C

Idioma: Ingles.
Referencias bibliográficas: 27
Paginas: 100-110
Archivo PDF: 501.67 Kb.


RESUMEN

La ruptura completa del ligamento cruzado anterior (LCA) es un problema común en el área de ortopedia. En la actualidad existen diversas técnicas para la reconstrucción de los ligamentos, las cuales incluyen el uso de autoinjertos, aloinjertos y en algunos casos ligamentos artificiales. Estos últimos no han demostrado buenos resultados a corto, mediano y largo plazo. El propósito del presente estudio es diseñar un tejido funcionalmente biológico capaz de ser usado para el reemplazo de los ligamentos de la rodilla aplicando técnicas de ingeniería de tejidos y estimulación mecánica con un biorreactor, promoviendo la diferenciación celular y la producción de matriz extracelular. En este estudio preliminar, el neotejido fue caracterizado con pruebas mecánicas y pruebas biológicas (viabilidad e inmunohistoquímica), comparando su comportamiento con el del tejido nativo. Las pruebas mecánicas y las pruebas biológicas demostraron que la estimulación mecánica dada por un biorreactor promueve el mantenimiento de la expresión fenotípica de fibroblastos de ligamento y la síntesis de matriz extracelular.


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