Martínez D, Cabrera L, Hernández AM

Idioma: Ingles.
Referencias bibliográficas: 45
Paginas: 2211-2216
Archivo PDF: 927.37 Kb.

RESUMEN

El P3 es un anticuerpo monoclonal murino de isotipo IgM, que reconoce a los gangliósidos N-glicolilados (un tipo de glicolípidos presente en la membrana celular de la mayoría de los vertebrados), a otros auto-antígenos como los sulfátidos y posee una alta homología con un anticuerpo que reconoce a la mielina de los oligodendrocitos. A pesar de ser una proteína autóloga, previamente se demostró que es capaz de activar una cascada idiotípica de células B y T CD4⁺ y CD8⁺ anti-idiotípicas en ausencia de adyuvantes o proteínas transportadoras. Las células B-1a son la población dominante durante las fases tempranas del desarrollo, cuando se establece la conectividad idiotípica, y los anticuerpos naturales de línea germinal secretados por estas células desempeñan un papel fundamental en la protección contra infecciones y el mantenimiento de la homeostasis. En este trabajo se demostró que el AcM P3 es capaz de reconocer a células B-1a de ratones BALB/c vírgenes. Este anticuerpo indujo en dichas células la expresión a altos niveles de los marcadores de activación CD25, CD69 y CD86, la secreción de IgM y un patrón mixto de las citocinas IFN-γ, IL-4 e IL-10. La capacidad del AcM P3 para activar células B-1a puede contribuir a su inmunogenicidad en el modelo singénico y, además, ser usada como una herramienta para direccionar específicamente contra las células B-1a y estimularlas.

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