medigraphic.com

2018, Número 3

Rev Educ Bioquimica 2018; 37 (3)


Estudio del transcriptoma mediante RNA-seq con énfasis en las especies vegetales no modelo

Rodríguez-Alonso G, Shishkova S

Idioma: Español
Referencias bibliográficas: 68
Paginas: 75-88
Archivo PDF: 720.74 Kb.


RESUMEN

Históricamente el desarrollo de la biología se ha limitado al estudio de unas pocas especies, a las cuales conocemos como especies modelo, y cuyas particularidades representan ventajas prácticas para su mantenimiento y estudio en el laboratorio. Sin embargo, la viabilidad actual para secuenciar el genoma y los transcriptomas de virtualmente cualquier especie permite la inclusión de nuevos organismos como modelo de estudio. En esta revisión se presenta una descripción general de las principales plataformas de secuenciación de transcriptomas (RNA-seq), así como los pasos básicos para el ensamblaje de transcriptomas cuando no se cuenta con un genoma de referencia. Finalmente, se proveen algunos ejemplos de estudios de transcriptoma aplicados a organismos no modelo.


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