Castro M, Fernandez E, Palma FP, Chaple GAM, Vildósola GP

Language: Spanish
References: 7
Page: 1-14
PDF size: 319.06 Kb.

ABSTRACT

Objective: Determine the antimicrobial properties of the incorporation of copper and zinc oxide nanoparticles into a total rinse and etch adhesive against Streptococcus mutans in patients with composite resin restorations made with loaded adhesive.
Methods: A randomized clinical study was conducted of 25 patients of both sexes from the orthodontics graduate course taught at the Dental School of the University of Chile, in whom the presence of Streptococcus mutans was confirmed in saliva. Occlusal composite resin restorations were performed in upper premolars with exodontia indicated as part of the orthodontic treatment, using loaded adhesive (composition 5 / 0.2% ZnO and Cu, respectively) and control (without nanoparticles in its composition), according to the randomization list. Microbiological samples were taken at three moments applying the tray technique (before, 1 week after and 4 weeks after the restorations). Streptococcus mutans were obtained, isolated and identified from the samples taken. Data analysis was based on the Mann-Whitney test.
Results: Mean Streptococcus mutans CFU count in the experimental group was higher after the composite resin restorations were made. Results of PCR molecular identification show the presence of Streptococcus mutans in 20 of 25 samples.
Conclusions: No differences were found in the Streptococcus mutans count before and after application of the adhesive over the composite resin restorations.

REFERENCES

1. Gutiérrez MF, Alegria-Acevedo LF, Mendez-Bauer L, Bermudez J, Davila-Sanchez A, Buvinic S, et al . Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles. J Dent. 2019 [acceso: 17/01/2020]; 82. Disponible en: Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S0300571219300211?via%3Dihub

2. Gutiérrez M, Malaquias P, Hass V, Matos T, Lourenço L, Reis A, et al . The role of copper nanoparticles in an etch-and-rinse adhesive on antimicrobial activity, mechanical properties and the durability of resin-dentine interfaces. J Dent. 2017 [acceso: 17/01/2020]; 61. Disponible en: Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S030057121730091X?via%3Dihub

3. Vildósola Grez P, Fernandez E, Cury Saad JR, Palma Fluxá P, Chaple Gil AM, Acuña Zepeda N, et al . Decrecimiento de Streptococcus mutans después de la aplicación de sellantes en superficies oclusales de molares permanentes en adultos. Rev Cubana Estomatol. 2019 [acceso: 17/01/2020]; 56(4). Disponible en: Disponible en: http://revestomatologia.sld.cu/index.php/est/article/view/2121

4. Vildósola P, Palma P, Zuñiga P, Fernandez E, Oliveira Junior OB, Moncada G. Recovering Streptococcus mutans over restorations by the tray technique: a randomized clinical study. Braz J Oral Sci. 2013 [acceso: 17/01/2020]; 12(4). Disponible en: Disponible en: http://www.scielo.br/scielo.php?pid=S1677-32252013000400004&script=sci_arttext

5. Huang B, Siqueira WL, Cvitkovitch DG, Finer Y. Esterase from a cariogenic bacterium hydrolyzes dental resins. Acta Biomater. 2018 [acceso: 17/01/2020]; 71. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/29496621

6. Mahshid S, Marjan R, Mohammadreza T, Amir P. Antimicrobial and bond strength properties of a dental adhesive containing zinc oxide nanoparticles. Braz J Oral Scie. 2016 [acceso: 17/01/2020]; 15(1). Disponible en: Disponible en: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8647127

7. Gutiérrez M, Malaquias P, Matos T, Szesz A, Souza S, Bermudez J, et al . Mechanical and microbiological properties and drug release modeling of an etch-and-rinse adhesive containing copper nanoparticles. Dent Mater. 2017 [acceso: 17/01/2020]; 33(3). Disponible en: Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S010956411630776X?via%3Dihub