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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2021, Number 3

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Rev Biomed 2021; 32 (3)

Evidence for Leptospira spp. in shrews Cryptotis mayensis. New host in Yucatan, Mexico

Suárez-Galaz AR, Hernández-Betancourt S, Panti-May JA, Manrique-Saide P, Torres-Castro M

Language: Spanish
References: 15
Page: 161-165
PDF size: 371.60 Kb.


ABSTRACT

Introduction. The pathogenic species of the Leptospira genus cause leptospirosis in susceptible humans and animals. Rodents are natural reservoirs of the bacteria; other wild animals are accidental hosts. Shrews have been involved in the transmission cycle of this pathogen in several countries; however, in Mexico, there is no information regarding this.
Objective. To describe the infection with Leptospira spp. in shrews captured in Yucatan, Mexico.
Material and methods. Two shrew specimens (Cryptotis mayensis) were captured in the municipality of Hunucma. Both were euthanized in order to collect samples from the kidney, spleen, and skeletal muscle for total DNA extraction. Leptospira spp. infection was screened by standard PCR for the amplification of a 16S ribosomal gene fragment (16S rRNA). Likewise, to verify and strengthen the results, other assays were carried out for the amplification of fragments corresponding to the LipL32 and rpoC genes.
Results. In the DNA extraction from kidney tissue from one specimen, amplifications of the 16S rRNA and rpoC genes were obtained.
Conclusion. This work represents the first report of Leptospira spp. infection in the renal tissue of C. mayensis from Yucatan, Mexico. More studies are needed in the region to determine the participation of these animals in the epidemiological cycle of this bacterial genus


REFERENCES

  1. Torres-Castro M, Hernández-Betancourt S, Agudelo- Flórez P, Arroyave-Sierra E, Zavala-Castro J, Puerto FI. Revisión actual de la epidemiología de la leptospirosis. Rev Med Inst Mex Seguro Soc. 2016 Sep-Oct; 54(5): 620-5. http://www.medigraphic.com/pdfs/imss/im- 2016/im165k.pdf

  2. Victoriano AF, Smythe LD, Gloriani-Barzaga N, Cavinta LL, Kasai T, Limpakarnjanarat K, et al. Leptospirosis in the Asia Pacific region. BMC Infect Dis. 2009 Sep; 9: 147. http://dx.doi.org/10.1186/1471-2334-9-147.

  3. Torres-Castro M, Cruz-Camargo B, Medina-Pinto R, Reyes-Hernández B, Moguel-Lehmer C, Medina R, et al. Detección molecular de leptospiras patógenas en roedores sinantrópicos y silvestres capturados en Yucatán, México. Biomedica. 2018 Aug; 38(S2): 51-8. http://dx.doi.org/10.7705/biomedica.v38i3.3938.

  4. Torres-Castro M, Febles-Solís V, Hernández-Betancourt S, Noh-Pech H, Estrella E, Peláez-Sánchez R, et al. Leptospira patógenas en murciélagos de Campeche y Yucatán, México. Rev MVZ Córdoba. 2020 May-Aug; 25(2): e1815. http://dx.doi.org/10.21897/rmvz.1815

  5. Ruiz-Piña HA, Puc-Franco MA, Flores-Abuxapqui J, Vado-Solis I, Cardenas-Marrufo MF. Isolation of Salmonella enterica and serologic reactivity to Leptospira interrogans in opossums (Didelphis virginiana) from Yucatán, México. Rev Inst Med Trop Sao Paulo 2002 Jul-Aug; 44(4): 235-7. http://dx.doi.org/10.1590/s0036- 46652002000400011

  6. Mayer-Scholl A, Hammerl JA, Schmidt S, Ulrich RG, Pfeffer M, Woll D, et al. Leptospira spp. in rodents and shrews in Germany. Int J Environ Res Public Health. 2014 Jul; 11(8): 7562-74. http://dx.doi.org/10.3390/ ijerph110807562

  7. Sikes RS; Animal Care and Use Committee of the American Society of Mammalogists. 2016 Guidelines of the American Society of Mammalogists for the use of wild mammals in research and education. J Mammal. 2016 Jun; 97(3): 663-88. http://dx.doi.org/10.1093/ jmammal/gyw078

  8. Chávez Á, Somarriba BF, Soto A, Sheleby-Elías J, Duttmann C, Jiménez E, et al. Detección de Leptospira spp. en animales y muestras ambientales de áreas peridomésticas en Nicaragua. Rev Panam Salud Publica. 2018 Mar; 42: e26. http://dx.doi.org/10.26633/ RPSP.2018.26

  9. Julio RGV, Agudelo-Flórez P, López JÁ, Sánchez RGP. New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA. Rev Soc Bras Med Trop. 2020 Mar; 53: e20190333. http://dx.doi. org/10.1590/0037-8682-0333-2019

  10. Mayer-Scholl A, Hammerl JA, Schmidt S, Ulrich RG, Pfeffer M, Woll D, et al. Leptospira spp. in rodents and shrews in Germany. Int J Environ Res Public Health. 2014 Jul; 11(8): 7562-74. http://dx.doi.org/10.3390/ ijerph110807562

  11. Nally JE, Arent Z, Bayles DO, Hornsby RL, Gilmore C, Regan S, et al. Emerging infectious disease implications of invasive mammalian species: the greater whitetoothed shrew (Crocidura russula) is associated with a novel serovar of pathogenic Leptospira in Ireland. PLoS Negl Trop Dis. 2016 Dec; 10(12): e0005174. http:// dx.doi.org/10.1371/journal.pntd.0005174

  12. Mgode GF, Katakweba AS, Mhamphi GG, Fwalo F, Bahari M, Mdangi M, et al. Prevalence of leptospirosis and toxoplasmosis: a study of rodents and shrews in cultivated and fallow land, Morogoro rural district, Tanzania. Tanzania J Hlth Res. 2014 Jul; 16(3): e11. http://dx.doi.org/10.4314/thrb.v16i3.11

  13. Ceballos G. Mammals of México. Baltimore: Johns Hopkins University Press; 2014.

  14. Valbuena-Torrealba C, Péfaur-Vega JE. Determinación de leptospirosis en roedores y marsupiales de la región Sur del Lago de Maracaibo, estado Mérida, Venezuela. Rev Científica. 2015 May-Jun; XXV(3): 193-9. http:// www.redalyc.org/pdf/959/95939206002.pdf

  15. Widiastuti D, Sholichah Z, Agustiningsih, Wijayanti N. Identification of pathogenic Leptospira in rat and shrew populations using rpoB gene and its spatial distribution in Boyolali District. Kesmas: National Public Health Journal. 2016 Aug; 11(1): 32-8. http:// dx.doi.org/10.21109/kesmas.v11i1.798




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