Rodríguez-Ortiz JF, Saucedo-Sariñana AM, Rosales-Reynoso MA, Barros-Núñez P

Language: Spanish
References: 20
Page: 166-171
PDF size: 157.35 Kb.

ABSTRACT

Introduction. The concentration and integrity of cell free DNA (cf- DNA) are diagnostic and prognostic biomarkers in multiple diseases.
Objective. To determine the concentration and integrity index of cf- DNA in peripheral blood of individuals from Jalisco, Mexico.
Method. Peripheral blood from 117 individuals was analyzed. cf- DNA was quantified by Qubit 2.0 fluorometry and by ALU115 q-PCR. Integrity index was determined by the ALU247/ALU115 ratio by q-PCR.
Results. The mean concentration of cf-DNA was 294 ng/ml by fluorometry and 108.08 ng/ml by q-PCR. Integrity index, by the ALU247/ALU115 ratio was 0.45. The concentration of cf-DNA showed a significant difference by age group, but not by sex. The integrity index did not show significant differences by sex or age groups.
Conclusions. There are wide differences in cf-DNA between the two procedures used here, as well as compared to other populations.

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