2020, Number 2
Chemical Screening and Antioxidant Activity of Couepia subcordata Benth. (Guaijí) Harvested in the Ecuadorian Coast Region
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ABSTRACT
Introduction: Couepia subcordata Benth. (Chrysobalanaceae), is a fruit of Amazonian origin, established in the coastal region of Ecuador and consumed fresh or processed in rural areas. Although important compounds such as triterpenes and antioxidants have been studied in wild relatives of the genus Couepia sp., little is known about other metabolites contained in this fruit and their medicinal properties.Objective: To characterize the volatile compounds, antioxidant capacity and the presence of ascorbic acid, coumarins, alkaloids, saponins, reducing sugars, phenols and flavonoids in Couepia subcordata.
Methods: About 50 mature fruits of C. subcordata were obtained in the city of Milagro and Naranjito, Ecuador. The pulp of each fruit was dried in an oven and subjected to alcoholic extractions and then analyzed for phytochemical profile by HPLC. Antioxidant capacity was determined by the DPPH (1,1- diphenyl-2-picrylhydrazyl) method and volatile compounds were analyzed by solid-phase microextraction followed by gas chromatography coupled to mass spectrometry.
Results: The results showed the significant presence of alkaloids, coumarins, low molecular weight flavonoids, reducing carbohydrates, and phenolic compounds (pyrocatechol tannins), while the levels of precipitates, saponins, and flavonoids were not detectable. The antioxidant capacity of the dehydrated pulp of C. subcordata (Guaijí) was 7.899 umol TE 100 g-1.
Conclusions: Couepia subcordata fruits provided new data associated with high contents of coumarins and alkaloids linked to antitumor, antiinflammatory, antiasthmatic and antioxidant activity. In addition, the fruit presented a richness of volatile compounds that can be used in the pharmaceutical and food industry. These results support efforts to characterize local fruits with potential to develop biodiversity valorization processes.
REFERENCES
Anunciação PC, Giuffrida D, Murador DC, De Paula Filho GX, Dugo G, Pinheiro-Sant’Ana HM. Identification and quantification of the native carotenoid composition in fruits from the Brazilian Amazon by HPLC–DAD– APCI/MS. Journal of Food Composition and Analysis. 2019;83:103296. DOI: https://doi.org/10.1016/j.jfca.2019.103296
Berto A, Ribeiro AB, Sentandreu E, de Souza NE, Mercadante AZ, Chisté RC, Fernandes E. The seed of the Amazonian fruit Couepia bracteosa exhibits a greater elimination capacity against ROS and RNS than their husk and pulp extracts. Food and function. 2015;6: 9-3081-3090. DOI: https://doi.org/10.1039/C5FO00722D
Cabrera G, Guaramato MA, Rodríguez M, Méndez J, Rodríguez Ortega M, Carvajal Z, González Mujica F. Couepia paraensis: Phytochemicals study, cytotoxicity and glucose-6-phosphatase inhibition tests. Latin American and Caribbean Bulletin of Medicinal and aromatic plants. 2012[acceso: 1/12/2019];11(3):241-8. Disponible en: https://www.redalyc.org/pdf/856/85622739006.pdf
Cardoso R, de Carvalho JEU, Barbosa W. Physical and physicochemical characterization of Marirana (Couepia subcordata Benth.). In Embrapa Eastern Amazonia-Summary in Annals of Congress (ALICE). Embrapa Eastern Amazonia. 2003[acceso: 10/10/2019];1:475. Disponible en: https://www.alice.cnptia.embrapa.br/bitstream/doc/575175/1/475.pdf
Castro KA, García AR. Elaboration of a yogurt with dehydrated pulp of Couepia subcordata (Guaijí), enriched with zinc salts and sweetened with stevia Dissertation bachelor's thesis, Universidad de Guayaquil, Facultad de Ingeniería Química. Guayaquil, Ecuador. 2017[acceso: 10/7/2019];35-6. Disponible en: http://repositorio.ug.edu.ec/handle/redug/18338
Fernandes KRP, Bittercourt PS, Souza ADL, Souza AQL, Silva FMA, Lima ES, Acho LDR, Nunomura RCS, Teixeira AF, Koolen HHF. Venous ferrule phenolic compounds (Myristicaceae) and evaluation of its antioxidant and enzyme inhibitor potential. Acta Amazonica. 2019;49:1-48-53. DOI: http://dx.doi.org/10.1590/1809-4392201800832
Lal NK, Butnaru R, Imionescu CS. Investigations in the field of chemical and morphological structure of certain tropical wood species. The study of their chemical composition. Cellulose chemistry and technology [1977]. 2013[acceso: 12/7/2020];1:59-66. Disponible en: https://agris.fao.org/agrissearch/ search.do?recordID=US201302978716
Massing LT, Mourão RHV, Bouillet LÉM, Bernardes RSA, Andrade EHA, Tremea A, Maia JGS. Nutritional composition of the pulp of Pajurá (Couepia bracteosa Benth.), an underutilized fruit from the Amazon. Integrative Food, Nutrition and Metabolism, 2018;5(2); 1-7. DOI: https://doi.org/10.15761/IFNM.1000213
Michajluk Barboza BJ, Bazán D, Mereles L, Degen R, Alvarenga N. Caracterización física, análisis fitoquímico, huella digital cromatográfica, capacidad antioxidante y actividad antimicrobiana de los frutos de Vitex megapotamica. Rev Cuba Plant Med. 2019[acceso:8/3/2020];24(1):[aprox.0p.]. Disponible en: http://revplantasmedicinales.sld.cu/index.php/pla/article/view/743
Paludo MC, de Oliveira LF, Hermosín Gutiérrez I, Ballus CA, Ribeiro AB, Pimentel SB, Godoy HT. Extracts of peels and seeds of five varieties of Brazilian Jabuticaba present high capacity to deactivate reactive species of oxygen and nitrogen. Plant Foods for Human Nutrition. 2019;74(1):135-40. DOI: https://doi.org/10.1007/s11130-019-0712-7
Paracampo NENP, Prance GT, Poppi RJ, Da Silva JAF. Chemotaxonomic study of Chrysobalanus icaco Linnaeus (Chrysobalanaceae) using ultra high performance liquid chromatography coupled with diode array detection fingerprint in combination with multivariate analysis. Journal of separation science. 2017;40(10):2161-2169. DOI: https://doi.org/10.1002/jssc.201601444