medigraphic.com
SPANISH

Revista Cubana de Plantas Medicinales

ISSN 1028-4796 (Print)
  • Contents
  • View Archive
  • Information
    • General Information        
    • Directory
  • Publish
    • Instructions for authors        
  • medigraphic.com
    • Home
    • Journals index            
    • Register / Login
  • Mi perfil

2020, Number 3

<< Back Next >>

Rev Cubana Plant Med 2020; 25 (3)

Design of an infusion of Moringa oleifera Lam. (moringa) and Hibiscus sabdariffa L. (Jamaican flower)

Campo FM, Burgos DKA, Reyes JMG, Matute CNL, Cun CJV, Cuesta-Rubio O, Márquez HI, Jaramillo JCG
Full text How to cite this article

Language: Spanish
References: 34
Page:
PDF size: 238.95 Kb.


Key words:

Moringa oleifera Lam, Hibiscus sabdariffa L, infusion, phenolic compounds, antioxidant activity, HPLC/MS.

ABSTRACT

Introduction: Currently, natural foods are sought that have nutritional and functional properties and that are easy to introduce to the diet of people. The combination of leaves of Moringa oleifera Lam., Moringaceae, with the chalices of Hibiscus sabdariffa L., Malvaceae, would achieve a beneficial infusion for health due to the content of water-soluble bioactive compounds that it provides.
Objective: To design a formulation to prepare as an infusion, combining the leaves of M. oleifera (moringa) with the chalices of H. sabdariffa (flower of Jamaica).
Methods: The raw materials were evaluated by determining residual humidity, total ash and insoluble in acid, minerals, protein and total phenols (Folin- Ciocalteu). By means of high-efficiency liquid chromatography / mass spectrometry (HPLC / MS), the major compounds were established and, in addition, the antioxidant capacity (IC50 by DPPH) was determined. Three formulations were designed, the one with the greatest sensory acceptance was chosen and the corresponding physical-chemical quality controls were performed on this.
Results: The quality standards determined to dry drugs are within the limits referred to in the literature. The chemical study allowed to identify glycosidic flavonoids, derivatives of quercetin, apigenin and kaempferol; phenolic acids and a lignan in the aqueous extract of the M. oleifera. In addition to organic acids and the amino acid phenylalanine. In the aqueous extract of the chalices of H. sabdariffa, anthocyanins, glycosidic flavonoids and organic acids were identified. The best accepted sensory formulation was the 50:50 mixtures of both drugs. The quality control of the infusion, through various physicochemical tests, allowed its standardization. The quantification of polyphenols in 200 mL of water was 49.34 mg EAG and the IC50 of DPPH of 0.06 mg / mL.
Conclusions: The designed infusion has the necessary characteristics to be considered, for its nutritional and antioxidant properties, as a beneficial beverage for human health.


REFERENCES

  1. Guaycha N, Jaramillo C, Cuenca S, Tocto J, Márquez I. Estudios farmacognósticos y toxicológicos preliminares de hojas, tallo y raíz de moringa (Moringa oleifera Lam.). Revista Ciencia. UNEMI. 2017[acceso:30/11/2018];10(22):60-8. Disponible en: http://ojs.unemi.edu.ec/index.php/cienciaunemi/article/view/459

  2. Leone A, Spada A, Battezzati A, Schiraldi A, Aristil J, Bertoli S. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. Int. J. Mol. Sci. 2015[acceso:30/11/2018];16(6):12791-835. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490473/

  3. Abdallah E. Antibacterial properties of leaf extracts of Moringa oleifera Lam. growing in sudan. Journal of Advances in Medical and Pharmaceutical Sciences (JAMP). 2016 [acceso:30/11/2018];5(1):1-5. Disponible en: https://pdfs.semanticscholar.org/39bc/e9f1d9288cca16c1a0383ab5b62eb09d3 e52.pdf

  4. Zhen J, Villani TS, Guo Y, Qi Y, Chin K, Pan MH, et al. Phytochemistry, antioxidant capacity, total phenolic content and anti-inflammatory activity of Hibiscus sabdariffa leaves. Food Chem. 2016;190:673-80. DOI: https://doi.org/10.1016/j.foodchem.2015.06.006

  5. Apáez-Barrios P, Pedraza-Santos ME, de las Nieves M. Rendimiento y concentración de antocianinas en Hibiscus sabdariffa L. con aplicación foliar de micronutrimentos. Rev. Chapingo Ser Hortic. 2018[acceso:5/1/2019];24(2):107-20. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1027- 152X2018000200107

  6. Salinas Y, Zúñiga A, Serrano V, Sánchez C. Color en cálices de Jamaica (Hibiscus sabdariffa L.) y su relación con características fisicoquímicas de sus extractos acuosos. Revista Chapingo. Ser Hortic. 2012[acceso:1/12/2018];18(3):395-407. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1027- 152X2012000300012

  7. AOAC. Official methods of analysis of the association of official’s analytical chemists, 17th edn. Association of Official Analytical Chemists, Arlington, Virginia. 2003[acceso:1/12/2018]:2-66. Disponible en: https://law.resource.org/pub/us/cfr/ibr/002/aoac.methods.1.1990.pdf

  8. Miranda M, Cuéllar A. Farmacognosia y productos naturales. Ciudad de La Habana. Cuba. MES. La Habana: Editorial Félix Varela; 2000.

  9. Bacteriological Analytical Manual (BAM). U.S. Food & Drug administration (FDA). [acceso:8/11/2018]. Disponible en: https://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm20 06949.htm

  10. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72(1-2):248-54. DOI: https://doi.org/10.1016/0003- 2697(76)90527-3

  11. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods Enzymol. 1999;299:152-178. DOI: https://doi.org/10.1016/S0076-6879(99)99017-1

  12. Campo M, Sojos CG, Bastidas EV, Silva KM, Matute NL, Cun JV, et al. Infusión de hojas de Moringa oleífera L (moringa) y cascarilla de Theobroma cacao L (cacao). Rev. Cub. Plantas Medicinales. 2019[acceso:1/4/2019];24(1). Disponible en: http://www.revplantasmedicinales.sld.cu/index.php/pla/article/view/803/3 57

  13. Barron-Yánez RM, García MR, Soto MR, Colinas T, Kite G. Flavonoides y actividad antioxidante de Calia secundiflora (Ort.) yakovlev. Rev Fitotec Mex. 2011[acceso:9/1/2019];34(3):151-7. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0187- 73802011000300005

  14. Rodríguez-Pérez C, Quirantes-Piné R, Fernández-Gutiérrez A, Segura- Carretero A. Optimization of extraction method to obtain a phenolic compounds-rich extract from Moringa oleifera Lam leaves. Ind Crops Prod. 2015;66:246–54. DOI: https://doi.org/10.1016/j.indcrop.2015.01.002

  15. Masike K, Khoza BS, Steenkamp PA, Smit E, Dubery IA, Madala NE. A Metabolomics-Guided Exploration of the Phytochemical Constituents of Vernonia fastigiata with the Aid of Pressurized Hot Water Extraction and Liquid Chromatography-Mass Spectrometry. Molecules 2017[acceso:10/1/2019];22:1200. Disponible en: https://www.mdpi.com/1420-3049/22/8/1200

  16. Makita C, Chimuka L, Steenkamp P, Cukrowska E, Madala E. Comparative Analyses of Flavonoid Content in Moringa oleifera and Moringa ovalifolia with the Aid of UHPLC-qTOF-MS Fingerprinting. S. Afr. J. Bot. 2016[acceso:1/12/2018];105:116–22. Disponible en: https://www.sciencedirect.com/science/article/pii/S0254629916002507

  17. Rodríguez-Medina IC, Beltrán-Debón R, Molina VM, Alonso-Villaverde C, Joven J, Menéndez JA, Segura-Carretero A, Fernández-Gutiérrez A. Direct Characterization of Aqueous Extract of Hibiscus Sabdariffa Using HPLC with Diode Array Detection Coupled to ESI and Ion Trap MS. J. Sep. Sci. 2009[acceso:12/1/2019];32(20):3441–8. Disponible en: https://pubmed.ncbi.nlm.nih.gov/19750503/

  18. Beltrán-Debón R, Alonso-Villaverde C, Aragones G, Rodriguez-Medina I, Rull A, Micol V, et al. The aqueous extract of Hibiscus sabdariffa calices modulates the production of monocyte chemoattractant protein-1 in humans. Phytomedicine. 2010[acceso:12/1/2019];17(3-4):86-191. Disponible en: https://pubmed.ncbi.nlm.nih.gov/19765963/

  19. Sarkar B, Kumar M, Mukhopadhyay K. A Rapid Method for Detection and Characterization of Anthocyanins from Hibiscus, Ocimum and Syzygium Species and Evaluation of Their Antioxidant Potential. In: Mukhopadhyay K, Sachan A, Kumar M. (eds) Applications of Biotechnology for Sustainable Development. Springer Singapore. 2017[acceso:11/1/2019]. Disponible en: https://link.springer.com/chapter/10.1007/978-981-10-5538-6_16

  20. Instituto Ecuatoriano de Normalización. Hierbas aromáticas. Requisitos. NTE INEN. 2007[acceso:1/12/2018];2392:2013. Disponible en: https://archive.org/details/ec.nte.2392.2007

  21. Márquez I, Bastidas TL, Fernández GK, Campo M, Jaramillo CG, Rojas L. Estudio farmacognóstico preliminar de tallo y raíz de la especie Moringa oleifera Lam cosechada en Machala. Rev. Cub. Plantas Medicinales. 2017[acceso:11/1/2019];22(1):1-13. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028- 47962017000100013

  22. Nnam NM, Onyeke NG. Chemical composition of two varieties of sorrel (Hibiscus Sabdariffa L.), calyces and the drinks made from them. Plant Foods Hum. Nutr. 2003[acceso:11/1/2019];58(3):1–7. Disponible en: https://link.springer.com/article/10.1023/B:QUAL.0000040310.80938.53

  23. Tazoho GM, Gouado I, Ndomou M, Bonsi ST, Wamba YM, Agbor EE. Clinical, hematological and biochemical health benefit effects of Hibiscus Sabdariffa Lin dried calyces beverage in human. Food Nutr. Sci. 2016;7(5):383–95. DOI: http://dx.doi.org/10.4236/fns.2016.75040

  24. Apáez-Barrios P, Pedraza-Santos ME, de las Nieves M. Yield and anthocyanin concentration in Hibiscus sabdariffa L. with foliar application of micronutrients. Rev. Chapingo Ser Hortic. 2018[acceso:11/1/2019];24(2):107- 20. Disponible en: http://www.scielo.org.mx/pdf/rcsh/v24n2/2007-4034- rcsh-24-02-107.pdf

  25. Gharibzahedi SMT, Jafari SM. The importance of minerals in human nutrition: Bioavailability, food fortification, processing effects and nanoencapsulation. Trends in Food Science & Technology 2017;62:119-32. DOI: https://doi.org/10.1016/j.tifs.2017.02.017

  26. Kirmani MZ, Mohiuddin S, Naz F, Naqvi I, Zahir E. Determination of some toxic and essential trace metals in some medicinal and edible plants of Karachi city. Journal of Basic & Applied Sciences. 2011[acceso:15/1/2019];7(2):89-95. Disponible en: https://www.researchgate.net/publication/267934323_Determination_of_so me_toxic_and_essential_trace_metals_in_some_medicinal_and_edible_plants _of_Karachi_city?enrichId=rgreq-f92d8c63dacafcc2b8d644d9f6669530- XXX&enrichSource=Y292ZXJQYWdlOzI2NzkzNDMyMztBUzoyOTkzNjU3MjI4MDQy MjRAMTQ0ODM4NTc1ODI4NA%3D%3D&el=1_x_2&_esc=publicationCoverPdf

  27. Londoño-Franco LF, Londoño-Muñoz PT, Muñoz-García FG. Los riesgos de los metales pesados en la salud humana y animal. Biotecnología en el Sector Agropecuario y Agroindustrial 2016[acceso:15/1/2019];14(2):145-53. Disponible en: http://www.scielo.org.co/pdf/bsaa/v14n2/v14n2a17.pdf

  28. Martínez-Flórez S, González-Gallego J, Culebras JM, Tuñón M. Los flavonoides: propiedades y acciones antioxidantes. Nutr. Hosp. 2012[acceso:15/1/2019];17(6):271-8. Disponible en: http://www.nutricionhospitalaria.com/pdf/3338.pdf

  29. Williamson E, Driver S, Baxter K. (Eds.). Stockley's herbal medicines interactions. Pharmaceutical Press. 2013. Disponible en: https://www.stonybrookmedicine.edu/sites/default/files/herbal_medicines_i nteractions-1.pdf

  30. Nobossé P, Fombang EN, Mbofung CM. Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves. Food Science & Nutrition. 2018[acceso:15/1/2019];6(8):2188-98. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261213/

  31. Vargas-Álvarez D, Chino-Patricio P, Damián-Nava A, Palemón-Alberto F, Hernández-Castro E, Silva-González M. Quercetin, kaempferol and apigenin in roselle (Hibiscus sabdariffa L.). Int. J. Adv. Res. Biol. Sci. 2018[acceso:15/1/2019];5(1):62-66. Disponible en: https://www.ijarbs.com/pdfcopy/jan2018/ijarbs11.pdf

  32. Bramati L, Aquilano F, Pietta P. Unfermented rooibos tea: quantitative characterization of flavonoids by HPLC− UV and determination of the total antioxidant activity. J. Agric. Food Chem. 2003;51(25):7472-74. DOI: https://doi.org/10.1021/jf0347721

  33. Reyes-Luengas A, Salinas-Moreno Y, Ovando-Cruz ME, Arteaga-Garibay RI, Martínez-Peña MD. Análisis de ácidos fenólicos y actividad antioxidante de extractos acuosos de variedades de Jamaica (Hibiscus sabdariffa L.) con cálices de colores diversos. Agrociencia. 2015[acceso:16/1/2019];49(3):277- 90. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405- 31952015000300004

  34. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol. 1995[acceso:16/1/2019];28(1):25-30. Disponible en: http://radio.cuci.udg.mx/bch/EN/Manuals/Techniques/DPPHoriginal_LebensWissTechnol_1995-v28-p25.pdf




2020     |     www.medigraphic.com

Mi perfil

C?MO CITAR (Vancouver)

Rev Cubana Plant Med. 2020;25