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2020, Number 4

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Rev Cubana Plant Med 2020; 25 (4)

Variation of Phenolic Content and Antioxidant Activity in Organs and Populations of Phlomis crinita L.

Merouane A, Fellag S, Noui A

Language: English
References: 26
Page:
PDF size: 205.03 Kb.


ABSTRACT

Introduction: The medicinal plant Phlomis crinita L. is widely used in Mediterranean folk medicine for its various pharmacological effects, especially to heal wounds and relieve abdominal pain.
Objective: Determine the phenolic and flavonoid content of hydromethanolic extracts obtained from Phlomis crinita L. leaves and flowers, as well as their antioxidant properties.
Methods: Total phenolic and flavonoid content was determined by spectrophotometry with the Folin-Ciocalteu and aluminum chloride methods. Antioxidant activity was estimated by the β-carotene-linoleic acid system and the 2,2-diphenyl-1-picrylhydrazyl free radical assay.
Results: Phytochemical examination showed that the content of bioactive compounds was clearly greater in leaf extracts than in flower extracts. The content of phenolic compounds varied between the three populations (p< 0.05), with the Ouled Benabdelkader population exhibiting the largest content (117.96 ± 1.70 μg GAE/mg). The greatest flavonoid content was found in leaves of Medjadja, followed by Ouled Benabdelkader and el-Nakhla (p < 0.05). The leaf extracts with the strongest antioxidant activity were the ones from the Ouled Benabdelkader population, of great importance in terms of DPPH free radical scavenging capacity. Maximum effect was achieved with a CI50= 73.80 ± 1.58 μg/ml. This potency is highly correlated with flavonoid content.
Conclusions: The study revealed the richness of P. crinita in bioactive compounds characterized by great antioxidant properties which make them a potentially valuable natural remedy.


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