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2021, Number 2

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Rev Mex Patol Clin Med Lab 2021; 68 (2)

Use of Hank's salts, saline and phosphate buffer (PBS) as transport medium in the identification of SARS-CoV-2 by RT-PCR

Parra-Ortega I, Carbajal-Franco E, Nieto-Rivera B, Galaviz-Hernández S, López-Martínez I, Barrera-Badillo G, López-Martínez B

Language: Spanish
References: 14
Page: 51-55
PDF size: 278.51 Kb.


ABSTRACT

Introduction: Early and timely detection of cases with the SARS-CoV-2 virus allows the implementation of isolation measures, which are aimed at mitigating the transmission of cases in this pandemic. Therefore, we show the evidence obtained in the handling of some alternatives as a means of transport for nasopharyngeal swab samples for the identification of SARS-CoV-2. Material and methods: Three transport media were prepared: 1) Hank's balanced salt solution, 2) 0.9% physiological saline, and 3) 1X phosphate buffer (PBS). The three media were supplemented with 5% heat inactivated fetal bovine serum and 1% penicillin/streptomycin. In the period May-July 2020, samples from all pediatric patients of the Hospital Infantil de México "Federico Gómez" who were tested for the detection of COVID-19 infection were included. In the analytical process of all cases, the values of the cycle threshold (Ct) of the viral genes and the internal control were recorded for the purposes of this study, only the value of the Ct of the human RNAase P gene (internal control) was used, to evaluate the presence of genetic material obtained in the sampling. Results: No differences were documented between the different means of viral transport, both in positive and negative cases. Conclusions: The use of viral transport media elaborated in diagnostic laboratories like ours are of great help for the identification of the SARS-CoV-2 virus without compromising the quality of the results, as long as the pertinent analysis and monitoring is carried out performance at all stages of the process and establish control points at each stage.


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