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2021, Number 2

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Rev Mex Patol Clin Med Lab 2021; 68 (2)

Advantages and disadvantages of 5 detection kits of SARS-CoV-2 by RT-qPCR. Experience of the Hospital de Niños ''Dr. Roberto del Río'' clinical laboratory

Ovalle R, Morales P, Clement P, Rojas-Aedo JF

Language: Spanish
References: 7
Page: 59-61
PDF size: 192.90 Kb.


ABSTRACT

During 2020, the clinical laboratory of "Dr. Roberto del Río" Children's Hospital carried out detection tests for SARS-CoV-2 for clinical diagnosis during pandemics, with five different detection kits that showed differences in sensitivity, workflow, interpretation of results and presence of false positives. After using each kit for at least 45 days, the LightCycler Multiplex RNA Virus Master kit with LightMix modular SARS-CoV-2 (COVID-19) RdRp and Modular Dx kit EAV Extraction Control (Roche) was found to be the most convenient kits to use, presenting the best general performance (running time, workflow, and inhibition). In contrast, the less convenient kit was Allplex 2019-nCoV Assay (Seegene), due to a high inhibition rate and long running time.


REFERENCES

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