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ISSN 1405-8790 (Print)

2003, Number 3-4

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Rev Mex Med Fis Rehab 2003; 15 (3-4)

Low Laser stimulation (l = 650 nm) and cultivated fibroblasts proliferation in human tissues

Lomelí-Rivas A, Krötzsch E, Michtchenko A

Language: Spanish
References: 10
Page: 69-71
PDF size: 58.07 Kb.


ABSTRACT

Low-level laser stimulation has been used successfully in the treatment of wound healing, and other diseases. Fibroblasts are cells involved in tissue repair, it is needed adequate laser doses for fibroblasts stimulation. The objective of this study is to know the effects of λ = 650 nm laser in proliferation of human cultivated fibroblasts, utilizing clinical doses. Human fibroblasts were synchronized in G0 stage, and irradiated with continuous wave of a 650 nm low-level laser at doses of 0.5, 1, 3, 5 and 7 J/cm2, with power density of 30 mW/cm2. Fibroblast proliferation was measured with a [3H]-tymidine incorporation method. The results were measured at 24, 48, 72 and 96 hours. Fibroblast proliferation showed no statistical differences between controls, but it was observed some structure changes. It is probably that clinical doses are not appropriated for culture stimulation, or maybe, it is needed another power density.


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