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ISSN 1561-3186 (Electronic)

2021, Number 2

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Rev Acta Médica 2021; 22 (2)

Use of the GENVINSET HLA Celiac Kit in Cobas z480 Analyzer for HLA DQ Molecules Detection

García MG, Horta RM, González GN, Ortega CA, Hernández AB, Chang MA

Language: Spanish
References: 20
Page:
PDF size: 248.84 Kb.


ABSTRACT

Objective: To evaluate the compatibility of the GENVINSET HLA CELIAC (Blackhills Diagnostic Resources, BDR) system for the determination of HLA class II alleles: HLA-DQ2 (DQB1*02 and DQA1*05) and / or HLA-DQ8 (DQB1* 03:02), an important tool for the diagnosis of celiac disease, with the Cobas z 480 (Roche) equipment.
Methods: Three DNA samples were identified as controls, which were previously typed with low resolution Olerup SSP® HLA-A-B-DR-DQ system and high resolution Olerup SSP® DQB1 * 03 system. Thirty-three DNA samples from individuals with a probable diagnosis of celiac disease were analyzed, based on histological results obtained from a small bowel biopsy.
Results: The analysis of 33 samples showed that 23 were positive for at least one HLA-DQ allele and 10 were negative. The predominant frequency of HLADQ was associated to HLA-DQB1*02 (52%), followed by the combination of HLADQB1* 02/DQA1*05 (9%) and HLA-DQB1*03:02 (9%).
Conclusion: The commercial kit GENVINSET HLA CELIAC (BDR) can be used in Cobas z 480 Real Time PCR platform (Roche).


REFERENCES

  1. Dickson BC, Streutker CJ, Chetty R. Coeliac disease: an update forpathologists. J Clin Pathol. 2006;59:1008–16. DOI:http://dx.doi.org/10.1136/jcp.2005.035345.

  2. Tovoli F, Masi C, Guidetti E, Negrini G, Paterini P, Bolondi L. Clinical anddiagnostic aspects of gluten related disorders. World J Clin Cases.2015;3(3):275-84. DOI: http://dx.doi.org/10.12998/wjcc.v3.i3.275

  3. Ludvigsson JF, Rubio-Tapia A, van Dyke CT, Melton LJ 3rd, Zinsmeister AR,Lahr BD, Murray JA. Increasing incidence of celiac disease in a North Americanpopulation. Am J Gastroenterol. 2013;108(5):818-24. DOI:http://dx.doi.org/10.1038/ajg.2013.60

  4. Tye-Din JA, Galipeau HJ, Agardh D. Celiac Disease: A Review of CurrentConcepts in Pathogenesis, Prevention, and Novel Therapies. Front Pediatr.2018;6:350. DOI: http://dx.doi.org/10.3389/fped.2018.00350

  5. Caio G, Volta U, Sapone A, Leffler DA, De Giorgio R, Catassi C, et al. Celiacdisease: a comprehensive current review. BMC Medicine. 2019;17(1):142. DOI:http://dx.doi.org/10.1186/s12916-019-1380-z

  6. Kaukinen K, Partanen J, Mäki M, Collin P. HLA-DQ typing in the diagnosis ofceliac disease. Am J Gastroenterol. 2002;97(3):695-9. DOI:http://dx.doi.org/10.1111/j.1572-0241.2002.05471.x

  7. Sharma N, Bhatia S, Chunduri V, Kaur S, Sharma S, Kapoor P, et al.Pathogenesis of Celiac Disease and Other Gluten Related Disorders in Wheat and Strategies for Mitigating Them. Front Nutr. 2020;7:6. DOI:http://dx.doi.org/10.3389/fnut.2020.00006

  8. Selleski N, Almeida LM, Almeida FC, Gandolfi L, Pratesi R, Nóbrega YK.Simplifying Celiac Disease Predisposing HLA-DQ Alleles Determination by theReal Time PCR Method. Arq Gastroenterol. 2015;52(2):143-6. DOI:http://dx.doi.org/10.1590/S0004-28032015000200013

  9. Molecular Diagnostic of Celiac Disease. GENVINSET LINE. GENVINSET HLACELIAC. Real Time PCR assay, Product Code GSV-DQ-48 ,GSV-DQ-24. CE-IVD,No of test 48. [acceso:25/3/2018]. Disponible en:www.blackhilldiagnostic.com

  10. RMD/Cobas ® 4800 System, SBN-RMD-2020-002 version 1. FT 19 FSNTemplate V2: 01, 2014. cobas z 480 analyzer.GMMI: 0500881001- RocheDiagnostics. [acceso:25/3/2018]. Disponible en:https://diagnostics.roche.com

  11. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specificpolymerase chain reaction product by utilizing the 5'-3' exonuclease activity ofThermus aquaticus DNA polymerase. Proc Natl Acad Sci U S A.1991;88(16):7276-80. DOI: http://dx.doi.org/10.1073/pnas. 88.16.7276

  12. Farina F, Picascia S, Pisapia L, Barba P, Vitale S, Franzese A, Mozzillo E,Gianfrani C, Del Pozzo GG. HLA-DQA1 and HLA-DQB1 Alleles, ConferringSusceptibility to Celiac Disease and Type 1 Diabetes, are More Expressed ThanNon-Predisposing Alleles and are Coordinately Regulated. Cells. 2019 Jul19;8(7):751. DOI: http://dx.doi.org/10.3390/cells8070751

  13. Mashayekhi K, Rostami-Nejad M, Amani D, Rezaei-Tavirani M, Mohaghegh-Shalmani H, Zali MR. A rapid and sensitive assay to identify HLA-DQ2/8 riskalleles for celiac disease using real-time PCR method. Gastroenterol HepatolBed Bench. 2018[acceso:25/3/2018];11(3):250-8. Disponible en:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040037/

  14. Paradoa M, Middleton D, Acosta A, Sarmiento ME, Leyva J. Genes HLA enuna muestra de la población cubana. Vaccimonitor.2000[acceso:25/3/2018];9(3):1-5. Disponible en:http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2000000300001&lng=es&nrm=iso.

  15. Morera Barrios LM, Ustáriz García C, García García MA, HernándezHernández A, Lam Díaz RM, Guerreiro Hernández AM, et al. Frecuencia deantígenos HLA en la población cubana, según características étnicas. Rev Cubana Hematol Inmunol Hemoter. 2005[acceso:25/3/2018];21(2). Disponibleen: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-02892005000200004&lng=es

  16. Alegre R, Moscoso J, Martínez J, Martin M, Suárez J, Moreno A. HLA genesin Cubans and the detection of Amerindian alleles. Mol Immunol.2007;44(9):2426-35. DOI: http://dx.doi.org/10.1016/j.molimm.2006.10.017

  17. Morera-Barrios LM, Chang-Monteagudo A, García-García MA, De la GuardiaO, Ustariz-García C, Marcell-Rodriguez L, et al. Frecuencia de genes HLA enpacientes con insuficiencia renal crónica procedentes del occidente y centrode Cuba. Revista Cubana de Hematol, Inmunol y Hemoter.2015[acceso:25/3/2018];31(1):32-40. Disponible en:http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-02892015000100004&nrm=iso

  18. Catassi C. El mapa mundial de la enfermedad celíaca. ActaGastroenterológica Latinoamericana. 2005[acceso:25/3/2018];35(1):46-55.Disponible en: http://www.redalyc.org/articulo.oa?id=19317328008

  19. Gujral N, Freeman HJ, Thomson AB. Celiac disease: prevalence, diagnosis,pathogenesis and treatment. World J Gastroenterol. 2012 Nov 14;18(42):6036-59. DOI: http://dx.doi.org/10.3748/wjg.v18.i42.6036

  20. Lau MS, Sanders DS. Optimizing the diagnosis of celiac disease. Curr OpinGastroenterol. 2017;33(3):173-80. DOI:http://dx.doi.org/10.1097/MOG.0000000000000343




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