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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán
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2022, Number 3

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Rev Biomed 2022; 33 (3)

Pooling of nasopharyngeal swabs to identify asymptomatic cases of SARS-CoV-2 virus during first wave of the COVID-19 pandemic

Granja-Pérez P, Ayora-Talavera G, Villanueva-Jorge S, Flores-Quintal F, Avilés-Gómez LE
Full text How to cite this article

Language: English
References: 8
Page: 138-142
PDF size: 302.68 Kb.


Key words:

Pooled samples, COVID-19, asymptomatic, first wave.

ABSTRACT

Introduction. Public and private health services worldwide have faced difficulties under the COVID-19 pandemic. High numbers of asymptomatic carriers difficulted the surveillance and tracing of infectious individuals. Also, diagnostic laboratories suffered shortage of reagents to perform on-time diagnosis. Pooling of clinical samples offers an alternative to overcome reagents unavailability and diagnosis of asymptomatic individuals.
Methods. We performed pooling of 1937 clinical samples from asymptomatic people performing essential activities during the first wave of the COVID-19 pandemic in Yucatan, Mexico. Diagnosis by real time RT-PCR using the Berlin Method was performed in 229 pools.
Results. The strategy successfully detected asymptomatic COVID-19 positive cases. A total of 27 pools were positive. Individuals performing essential activities as government administrative, food markets and police force were the most commonly positive.
Conclusion. Pooling clinical samples is a strategy for the on-time isolation of infected individuals as well for saving laboratory resources.


REFERENCES

  1. CDC Centers for Diseases Control and Report. InterimGuidance for Use of Pooling Procedures in SARSCoV-2 Diagnostic, Screening, and Surveillance Testing| CDC [Internet]. Vol. 2019, Cdc. 2020. p. 1–8.

  2. Pasomsub E, Watcharananan SP, Watthanachockchai T,Rakmanee K, Tassaneetrithep B, Kiertiburanakul S, etal. Saliva sample pooling for the detection of SARSCoV-2. J Med Virol. 2021;93(3):1506–11. doi:10.1002/jmv.26460

  3. Herrera LA, Hidalgo-Miranda A, Reynoso-NoverónN, Meneses-García AA, Mendoza-Vargas A, Reyes-Grajeda JP, et al. Saliva is a reliable and accessiblesource for the detection of SARS-CoV-2. Int J Infect Dis.2021;105:83–90. doi:10.1016/j.ijid.2021.02.009

  4. de Salazar A, Aguilera A, Trastoy R, Fuentes A, AladosJC, Causse M, et al. Sample pooling for SARS-CoV-2 RTPCRscreening. Clin Microbiol Infect. 2020;26(12):1687.e1-1687.e5. doi:10.1016/j.cmi.2020.09.008

  5. Abdalhamid B, Bilder CR, McCutchen EL, Hinrichs SH,Koepsell SA, Iwen PC. Assessment of Specimen Poolingto Conserve SARS CoV-2 Testing Resources. Am J ClinPathol. 2020;153(6):715–8. doi:10.1093/ajcp/aqaa064

  6. Corman VM, Landt O, Kaiser M, Molenkamp R, MeijerA, Chu DKW, et al. Detection of 2019 novel coronavirus(2019-nCoV) by real-time RT-PCR. Eurosurveillance.2020;25(3):pii=2000045. doi:10.2807/1560-7917.ES.2020.25.3.2000045

  7. G. Ayora-Talavera, P. Granja-Perez, M. Sauri-Vivas, C.I.Hernández-Fuentes, I.P. Hennessee, I. López-Martínez,G. Barrera-Badillo, A. Che-Mendoza, P. Manrique-Saide, J.A. Clennon, H. Gómez-Dantés GV-P. Impactof layered non-pharmacological interventions onCOVID-19 tranmission dynamics in Yucatan, Mexico.Prev Med Reports. 2022;28:101843. doi:Https://doi.org/10.1016/j.pmedr.2022.101843

  8. Bilder CR, Iwen PC, Abdalhamid B. Pool Size SelectionWhen Testing for Severe Acute Respiratory SyndromeCoronavirus 2. Clin Infect Dis. 2021;72(6):1104–5.doi:10.1093/cid/ciaa774




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Rev Biomed. 2022;33