Díaz-Soto MT, Valdés GL, Bécquer MÁ, Oyarzábal YA, Alonso CI, Calderín-Miranda JM

Language: Spanish
References: 11
Page: 1-10
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ABSTRACT

Introduction: Oxidative stress is an entity with marked participation in the development of neuronal damage in the case of addictions, mainly in alcoholism. However, most reported clinical and non-clinical studies explore the stages of alcoholism and abstinence, so early-stage studies are insufficient.
Objective: Evaluate the brain redox status of Wistar rats in a social drinker model.
Methods: Redox markers of protection (superoxide dismutase, catalase, reduced glutathione) and damage (malondialdehyde) were determined in a social drinker model in Wistar rats.
Results: A redox imbalance was observed at the brain level, evidenced by the variation of antioxidant enzymes and malondialdehyde´s concentration levels in the ethanol consuming group.
Conclusions: The cerebral redox imbalance observed in Wistar rats, in a social drinker model, is representative of an incipient lipid peroxidation that shows that in the non-clinical model of social drinker there is neuronal damage.

REFERENCES

1. Duquesne-Chávez A, Díaz-Soto MT. Evaluaciones Conductuales en un modelode bebedor social en ratas Wistar. Rev. CENIC, Cienc. biol. 2020 [acceso10/12/2020];51(3):164-72. Disponible en:https://revista.cnic.cu/index.php/RevBiol/article/view/455

2. Jesse S, Bråthen G, Ferrara M, Keindi M, Ben-Menachem E, Tenasescu R, et al.Alcohol withdrawal syndrome: mechanisms, manifestations, and management.Acta Neurol Scand. 2017;135;(1):4-16. DOI: 10.1111/ane.12671

3. Díaz-Soto MT, Calderín-Miranda JM. Síndrome de abstinencia alcohólica:Resultado del estrés oxidativo y desequilibrio neuronal. Estado del arte. RevBiomed. 2020;31(2):96-107. DOI: 10.32776/revbiomed.v31i2.779

4. Díaz-Soto MT, Fraga-Pérez A, Dranguet-Vaillant J, Jerez E, Bécquer M,Casanova M, et al. Efectos del ozono médico sobre la Peroxidación Lipídica y laactividad de la Aldehído deshidrogenasa 2 cerebral en ratas alcohólicas enabstinencia. Rev farmacol Chile. 2019 [acceso 14/11/2020];12(12):30-40.Disponible en: https://www.sofarchi.cl/wp-content/uploads/Revista-de-Farmacologi%CC%81a-de-Chile-2019-V12-N%C2%B02.pdf

5. Boehringer Mannheim. Biochemica Information. A revised biochemicalreference source. Enzymes for routine. 1.a ed. Berlin: Boehringer Mannheim;1987: 15-16.

6. Sun Y, Oberley L, Li Y. A simple method for clinical assay of superoxidedismutase. Clin Chem. 1988;34(3):497-500. DOI: 10.1093/clinchem/34.3.497

7. Ecobichon DJ. Glutathione despletion and resynthesis in laboratory animals.Drug Chem Toxicol. 1984;7(4):345-55. DOI: 10.3109/01480548408998263

8. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidationproduct: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990;186:407-21. DOI: 10.1016/0076-6879(90)86134-h

9. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and non-sulfhydrylgroups in tissue with Ellman’s reagent. Anal Biochem. 1968;25(1):192-205. DOI:

10. 10.1016/0003-2697(68)90092-410. Asociación Médica Mundial. Declaración de la AMM sobre el Uso de Animalesen la Investigación Biomédica. Buenos Aires, Argentina: WMA; 2016 [acceso09/02/2020]. Disponible en: https://www.wma.net/es/policiespost/declaracionde-la-amm-sobre-el-usode-animales-en-lainvestigacionbiomedica/

11. León-Regal M, Cedeño-Morales R, Rivero-Morey R, Rivero-Morey J, García-Pérez D, Bordón-González L. La teoría del estrés oxidativo como causa directadel envejecimiento celular. Medisur. 2018 [acceso 10/01/2021];16(5). Disponibleen: http://medisur.sld.cu/index.php/medisur/article/view/3798.