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Órgano Oficial de la Asociación Dental Mexicana

2003, Number 3

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Rev ADM 2003; 60 (3)

Effects of mechanical stress in human osteoblasts under organ culture condition with respect to interleukin 1β production

García LS, Villanueva RE, Bojalil PR, Montaño LF, Massó LF, Ramírez AV

Language: Spanish
References: 28
Page: 85-89
PDF size: 76.86 Kb.


ABSTRACT

Mechanical bone remodelling is utilized by orthodontists, when forces are exerted on tooth roots that enable to move through alveolar bone. Such remodelling involves the activation of bone cells and the simultaneous breakdown and reformation of the bone matrix. Mechanical forces are applied in the correction of dentofacial discrepancies and dentoalveolar malocclusions during orthodontic tooth movement, nevertheless the cellular mechanism involved in the translation of biomechanics forces into molecular events are poorly understood.The aim of this study was to assay the effects of mechanical stress in human osteoblasts-like cells (Saos-2) under organ culture condition with respect to Interleukin 1β (IL-1β) production. In this report we have developed a model to deforming osteoblasts in monolayer cultures calpable of deformation. We examined the behaviour of Saos-2 cells with respect to cytokine production, when stimulated by a cycle of cell deformation of 5 seconds every 1.5 minutes after 8, 24, 48 and 72 hours. The cellular perturbation in these cells was then compared to underformed cells (n = 5). Cells were grown to confluence in Ham’s F12/DMEM media which contained 10% foetal calf serum, 100 µg/mL streptomycin, 100 U/mL penicillin and 0.25 µg/mL amphotericin and grown in tissue culture flask at 37oC. In a humidified atmosphere 95% air – 5% CO2. Enzyme-linked immunoassay (ELISA) was used to measure Interleukin-1β production. Results have shown that human clonal osteoblasts do not produce IL-1β, at 8 hours nevertheless at 24 hours (13.5 ± 2.1), 48 (23.2 ± 1.3) and 76 hours (33.9 ± 1.9) there is a significantly increased production of IL-1β compared to the control (p ‹ 0.0001). These results suggests that human monoclonal osteoblasts-like cells were influenced by mechanical strain in vitro and may account for some of the cellular effects associated with bone remodelling and orthodontic tooth movement.


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