medigraphic.com
ISSN 1561-3011 (Electronic)

2021, Number 3

<< Back Next >>

Rev Cubana Invest Bioméd 2021; 40 (3)

Antibacterial activity of a Peruvian propolis ethanolic extract against Streptococcus mutans

Checalla CJL, Sánchez-Tito MA

Language: Spanish
References: 27
Page: 1-12
PDF size: 206.31 Kb.


ABSTRACT

Introduction: Due to their important antimicrobial properties, propolis by-products are potentially useful for the prevention and treatment of dental caries.
Objective: Evaluate the antibacterial activity of a Peruvian propolis ethanolic extract against Streptococcus mutans.
Methods: The propolis ethanolic extract was obtained by maceration in 70% alcohol for 15 days. The extract was diluted in distilled water to obtain concentrations of 75%, 50% and 25%. Antibacterial activity was evaluated by the disk diffusion test in brain heart agar medium inoculated with S. mutans ATCC® 25175™. Chlorhexidine (CHX) 0.12% was used as control. The Petri plates were incubated for 48 hours at 37ºC in microaerophilic conditions. The inhibition haloes were then measured with a Vernier caliper.
Results: All the concentrations of the propolis ethanolic extract displayed antibacterial activity against S. mutans: 25% = 17.582 ± 2.578 mm; 50% = 16.906 ± 1.892 mm; 75% = 16.881 ± 2.013 mm; 100% = 17.201 ± 1.305 mm. However, values were lower than those of 0.12% CHX: 24.543 ± 2.486 mm (p < 0.05). According to the Duraffourd scale, S. mutans was sensitive (+) and very sensitive (++) to all propolis ethanolic extract concentrations, and highly sensitive to 0.12% chlorhexidine (+++) (p < 0.05).
Conclusions: The Peruvian propolis ethanolic extract displays significant antibacterial activity against S. mutans. Such activity was evaluated as sensitive and very sensitive.


REFERENCES

  1. Chen X, Daliri EB, Kim N, Kim JR, Yoo D, Oh DH. Microbial Etiology and Prevention of Dental Caries: Exploiting Natural Products to Inhibit Cariogenic Biofilms. Pathogens. 2020;9(7):569. DOI: 10.3390/pathogens9070569

  2. Pitts NB, Zero DT, Marsh PD, Ekstrand K, Weintraub JA, Ramos-Gomez F, et al. Dental caries. Nat Rev Dis Primers. 2017;3:17030. DOI: 10.1038/nrdp.2017.30

  3. Mathur VP, Dhillon JK. Dental Caries: A disease which needs attention. Indian J Pediatr. 2018;85(3):202-6. DOI: 10.1007/s12098-017-2381-6

  4. Bonetti D, Clarkson JE. Fluoride varnish for caries prevention: Efficacy and implementation. Caries Res. 2016;50(Suppl 1):45-9. DOI: 10.1159/000444268

  5. Janket SJ, Benwait J, Isaac P, Ackerson LK, Meurman JH. Oral and systemic effects of Xylitol consumption. Caries Res. 2019;53(5):491-501. DOI: 10.1159/000499194

  6. Airen B, Sarkar PA, Tomar U, Bishen KA. Antibacterial effect of propolis derived from tribal region on Streptococcus mutans and Lactobacillus acidophilus: An in vitro study. J Indian Soc Pedod Prev Dent. 2018;36:48-52.

  7. Somaraj V, Shenoy RP, Shenoy Panchmal G, Kumar V, Jodalli PS, Sonde L. Effect of herbal and fluoride mouth rinses on Streptococcus mutans and dental caries among 12-15-year-old school children: A randomized controlled trial. Int J Dent. 2017;5654373. DOI: 10.1155/2017/5654373

  8. Abbasi AJ, Mohammadi F, Bayat M, Gema SM, Ghadirian H, Seifi H, et al. Applications of propolis in dentistry: A review. Ethiop J Health Sci. 2018;28(4):505-512. DOI: 10.4314/ejhs.v28i4.16

  9. Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey, propolis, and royal jelly: A comprehensive review of their biological actions and health benefits. Oxid Med Cell Longev. 2017;1259510. DOI: 10.1155/2017/1259510

  10. Przybylek I, Karpinski TM. Antibacterial Properties of Propolis. Molecules. 2019;24(11):2047. DOI: 10.3390/molecules24112047

  11. Siheri W, Alenezi S, Tusiimire J, Watson DG. The chemical and biological properties of propolis. Bee products - chemical and biological properties. 2017;16:137-78. DOI: 10.1007/978-3-319-59689-1_7

  12. Machado BA, Silva RP, Barreto G de A, Costa SS, Silva DF, Brandão HN, et al. Chemical Composition and Biological Activity of Extracts Obtained by Supercritical Extraction and Ethanolic Extraction of Brown, Green and Red Propolis Derived from Different Geographic Regions in Brazil. PLoS One. 2016;11(1):e0145954. DOI: 10.1371/journal.pone.0145954

  13. Granados-Pineda J, Uribe-Uribe N, García-López P, Ramos-Godinez MDP, Rivero-Cruz JF, Pérez-Rojas JM. Effect of pinocembrin isolated from Mexican brown propolis on diabetic nephropathy. Molecules. 2018;23(4):852. DOI: 10.3390/molecules23040852

  14. Okinczyc P, Szumny A, Szperlik J, Kulma A, Franiczek R, Zbikowska B, et al. Profile of polyphenolic and essential oil composition of Polish propolis, black poplar and aspens buds. Molecules. 2018;23(6):1262. DOI: 10.3390/molecules23061262

  15. de Mendonça IC, Porto IC, do Nascimento TG, de Souza NS, Oliveira JM, Arruda RE, et al. Brazilian red propolis: phytochemical screening, antioxidant activity and effect against cancer cells. BMC Complement Altern Med. 2015;15:357. DOI: 10.1186/s12906-015-0888-9

  16. Freires IA, Queiroz VCPP, Furletti VF, Ikegaki M, de Alencar SM, Duarte MCT, et al. Chemical composition and antifungal potential of Brazilian propolis against Candida spp. J Mycol Med. 2016;26(2):122-32. DOI: 10.1016/j.mycmed.2016.01.003

  17. Becerra BT, Calla-Poma R, Requena-Mendizabal M, Millones-Gómez P. Antibacterial effect of Peruvian propolis collected during different seasons on the growth of Streptococcus mutans. Open Dent J. 2019;13:327-31. DOI: 10.2174/1874210601913010327

  18. Mayta-Tovalino F, Sacsaquispe-Contreras SJ. Evaluación in vitro del efecto antibacteriano del extracto etanólico de propóleo de Oxapampa-Perú sobre cultivos de Streptococcus mutans (ATCC 25175) y Staphylococcus aureus (ATCC 25923). Rev Estomatol Herediana. 2010;20(1):19-24.

  19. Eguizábal AM, Moroni NH. Actividad antibacteriana in vitro del extracto etanólico de propóleo peruano sobre Streptococcus mutans y Lactobacillus casei. Odontol Sanmarquina. 2007;10(2):18-20.

  20. Duraffourd C, Hervicourt LD, Lapraz JC. Cuadernos de Fitoterapia Clínica. Barcelona: Masson; 1986.

  21. Devequi-Nunes D, Machado BAS, Barreto GA, Rebouças Silva J, da Silva DF, da Rocha JLC, et al. Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction. PLoS One. 2018;13(12):e0207676. DOI: 10.1371/journal.pone.0207676

  22. Barrientos L, Herrera CL, Montenegro G, Ortega X, Veloz J, Alvear M, et al. Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Braz J Microbiol. 2013;44(2):577-85.

  23. Cayo Rojas CF, Cervantes Ganoza LA. La actividad antibacteriana de Camellia sinensis comparada con propóleo frente al Streptococcus mutans. Rev Cubana Estomatol. 2020 [acceso: 25/6/2020]; 57(1):e2967. Disponible en: http://www.revestomatologia.sld.cu/index.php/est/article/view/2967/1728

  24. Aminimoghadamfarouj N, Nematollahi A. Propolis diterpenes as a remarkable bio-source for drug discovery development: A review. Int J Mol Sci. 2017;18(6):1290. DOI: 10.3390/ijms18061290

  25. Duarte S, Rosalen PL, Hayacibara MF, Cury JA, Bowen WH, Marquis RE, et al. The influence of a novel propolis on mutans streptococci biofilms and caries development in rats. Arch Oral Biol. 2006;51(1):15-22. DOI: 10.1016/j.archoralbio.2005.06.002

  26. Park YK, Koo MH, Abreu JA, Ikegaki M, Cury JA, Rosalen PL. Antimicrobial activity of propolis on oral microorganisms. Curr Microbiol. 1998;36(1):24-8. DOI: 10.1007/s002849900274

  27. Koo H, Rosalen PL, Cury JA, Park YK, Bowen WH. Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity. Antimicrob Agents Chemother. 2002;46(5):1302-9. DOI: 10.1128/aac.46.5.1302-1309.2002




2020     |     www.medigraphic.com