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2021, Number 3

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Rev Cubana Med Trop 2021; 73 (3)

In vitro cytotoxic and antiproliferative activity of an aqueous extract from Phyllanthus comosus

del Barrio AGC, Morier DLF, Marrero, RJ

Language: Spanish
References: 24
Page: 1-13
PDF size: 366.46 Kb.


ABSTRACT

Introduction: Ever since the onset of ancient medical practice, plants have been used to treat a variety of conditions, including infectious communicable diseases and cancer. A large number of reports are available about the biological properties of the genus Phyllantus.
Objective: Evaluate the cytotoxic and antiproliferative activity of an aqueous extract of Phyllanthus comosus on three cell lines: two of tumoral origin (SiHa and HeLa) and one of non-tumoral origin (Vero).
Methods: Cytotoxic activity was evaluated by the MTT method, and antiproliferative capacity by colony inhibition detection or clonogenic assay. Mean cytotoxic concentration (CC50), selective index and cell proliferation reduction percentage were some of the values taken into account.
Results: The cytotoxicity assay obtained similar CC50 values for both tumor cell lines, whereas the value for the Vero line was three times less toxic, with a selectivity index above three. The clonogenic assay revealed proliferation inhibition in the tumor cell lines, whereas no inhibition of colony forming capacity was observed in Vero cells.
Conclusions: The P. comosus extract is more cytotoxic for tumoral cell lines SiHa and HeLa than for non-tumor Vero cells. Additionally, inhibition of the formation of cell clones in both tumor cell lines is evidence of its antiproliferative and selective action, substantiating its in vitro antitumor potential.


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