Riverón FG, Acosta ST, Marín PLC, Zúñiga RY, Torres RB, Pérez RJ

Language: Spanish
References: 13
Page: 316-322
PDF size: 286.45 Kb.

ABSTRACT

Background: the relationship between the reduced and oxidized form of glutathione, GSH/ GSSG, is frequently used as an indicator of the cellular redox state. Under conditions where high levels of oxidizing species are generated, reduced glutathione requirements can be increased, and therefore, the cellular redox state can be affected.
Objective: to determine the relationship between the cellular redox state and systemic markers of inflammation.
Methods: a descriptive study was carried out in a series of 56 cases referred from the immunogenetics clinic, aged between 1 and 76 years old, of both sexes. Erythrocyte sedimentation rate and serum levels of C-reactive protein were determined as systemic markers of inflammation. The GSH/GSSG ratio was calculated from the intraerythrocyte concentrations of reduced glutathione and its oxidized form, which were determined by an HPLC-UV method.
Results: the average GSH/GSSG ratio was 7.9 (95 % CI: 6.4-9.4) and age did not influence this proportion. In the cases that had altered values of the inflammation markers, they showed a decrease in the GSH/GSSG ratio. Cellular redox status was negatively correlated with erythrocyte sedimentation values (r=0.41; p=0.017). However, this association does not appear when C-reactive protein levels are analyzed.
Conclusions: the patients who present alterations in the inflammation markers show changes in the cellular redox state. The combined study of these biomarkers allows a more comprehensive analysis of the health status of patients with inflammatory diseases.

REFERENCES

1. Lushchak VI. Glutathione Homeostasis and Functions: Potential Targets for Medical Interventions. J Amino Acids. 2012;20(2):1-26.

2. Aquilano K, Baldelli S, Ciriolo MR. Glutathione: new roles in redox signaling for an old antioxidant. Front Pharmacol. 2014;5(1):196.

3. Lorenzen I, Mullen L, Bekeschus S, Hanschmann EM. Redox regulation of inflammatory processes is enzimatically controlled. Oxid Med Cell Longev. 2017;20(1):8459402.

4. Bourgonje AR, Feelisch M, Faber KN, Pasch A, Dijkstra G, van Goor H. Oxidative Stress and Redox-Modulating Therapeutics in Inflammatory Bowel Disease. Trends Mol Med. 2020;26(11):1034-46.

5. Merino J. Utilidad diagnóstica de la velocidad de sedimentación globular. Rev Med Gen Integr[Internet]. 2002[citado 13 Nov 2020];39(7):[aprox. 4p.]. Disponible en: https://www.elsevier.es/es-revista-medicina-integral-63-articulo-utilidad-diagnostica-velocidad-sedimentacion-globular-13029997.

6. Simona LD, Cacho C, Leva P, Barrero J, Aguar P. Development of a HPLC-UV method for simultaneous determination of intracelullar glutathione species in human cells. J Anal Bioanal Tech. 2015;6(4):259.

7. Colombet I, Pouchot J, Kronz V, Hanras X, Capron L, Durieux P, Wyplosz B. Agreement between erythrocyte sedimentation rate and C-reactive protein in hospital practice. Am J Med. 2010;123(9):7-13.

8. Lauridsen C. From oxidative stress to inflammation: redox balance and immune system. Poult Sci. 2019;98(10):4240-6.

9. Gyawali P, Richards RS. Association of altered hemorheology with oxidative stress and inflammation in metabolic syndrome. Redox Rep. 2015;20(3):139-44.

10. Feijóo M, Túnez I, Ruíz A, Tasset I, Muñoz E, Collantes E. Biomarcadores de estrés oxidativo como indicadores de actividad en la enfermedad articular crónica. Reumatol Clin[Internet]. 2010[citado 6 Feb 2020];6(2):[aprox. 4p.]. Disponible en: https://www.reumatologiaclinica.org/es-biomarcadores-estres-oxidativo-como-indicadores-articulo-S1699258X09001260.

11. Bray C, Bell LN, Liang H, Haykal R, Kaiksow F, Mazza JJ, Yale SH. Erythrocyte Sedimentation Rate and C-reactive Protein Measurements and Their Relevance in Clinical Medicine. WMJ. 2016;115(6):317-21.

12. Gadotti AC, Lipinski AL, Vasconcellos F, Marqueze LF, Cunha E, Campos AC, et al. Susceptibility of the patient infected with sars-Cov2 oxidative stress and possible interplay with severity of disease. Free Radic Biol Med. 2021;165(1):184-90.

13. Ertürk C, Altay MA, Büyükdogan H, Çaliskan G, Erel Ö. Thiol/disulfide homeostasis as a novel indicator of oxidative stress during the treatment process of patients with septic arthritis. Jt Dis Relat Surg. 2020;31(3):502-8.