medigraphic.com
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2023, Number 2

<< Back Next >>

Rev Biomed 2023; 34 (2)

Use of PCR assay to detect Leishmania-DNA in saliva from patients suffering American Cutaneous Leishmaniasis

Oswaldo AN, Crisante GE, Rojas A

Language: English
References: 20
Page: 251-258
PDF size: 303.45 Kb.


ABSTRACT

Introduction: American cutaneous leishmaniasis (ACL) affects over one million people annually worldwide. Its spread enables Leishmaniaparasites invading the skin, mucosa, and body-fluids including saliva. Detection of Leishmania-DNA in saliva, has been proposed as a noninvasive method.
Objective: To evaluate the use of multiplex conventional PCR-assay in saliva to identify Leishmania-DNA from patients infected with ACL.
Materials and Methods: Individuals with ACL were selected to evaluate a PCR-based saliva tool to identify Leishmania-DNA, 18 with active lesions and 3 with scars of previous healed ulcers, evaluated 2 years post-treatment. Saliva from 3 negative controls were included for comparison. Saliva samples (1 mL-each) were collected to be processed before, during and after treatment.
Results: Amplification of Leishmania-DNA in saliva from patients with healed and active lesions, revealed Leishmania braziliensis in 78% pre-treated and 28% during and after the treatment.
Conclusions: PCR-assay resulted useful identifying L. braziliensis in saliva from ACL-patients.


REFERENCES

  1. Añez N, Cazorla D, Nieves E, Chataing B, Castro M,Yarbuh AL. Epidemiología de la leishmaniasis en elEstado Mérida, Venezuela. I.-Diversidad y dispersiónde especies flebotominas en tres pisos altitudinales y suposible role en la transmisión de la enfermedad. MemInst Oswaldo Cruz. 1988. 83(4):455-63. https://doi.org/10.1590/S0074-02761988000400011

  2. Añez N, Rojas A, Scorza-Dagert JV, Morales C.Successful treatment against American cutaneousleishmaniasis by intralesional infiltration of a genericantimonial compound-lidocaine combination. A followup study. Acta Trop. 2018. Sep;185:261-66. doi:10.1016/j.actatropica.2018.06.001.

  3. Costa JML, Saldanha ACR, Nascimento D, SampaioG, Carneiro F, Lisboa E, et al. Clinical modalities,diagnosis and therapeutic approach of the tegumentaryleishmaniasis in Brazil. Gaz Méd Bahia. 2009. 79(3):70-83. http://gmbahia.ufba.br/index.php/gmbahia/article/viewfile/1033/1009

  4. Weigle K, Saravia NG. Natural history, clinical evolution,and the host-parasite interaction in New World cutaneousleishmaniasis. Clin Dermatol. 1996. 14(5):433-50.https://doi.org/10.1016/0738-081X(96)00036-3

  5. Mebrahtu YB, Van Eys E, Guizani I, Lawyer P, PambaH, Koech D, et al. Human cutaneous leishmaniasiscaused by Leishmania donovani s.l. in Kenya.Trans RSoc Trop Med Hyg, 1993. 87(5):598-601, https://doi.org/10.1016/0035-9203(93)90101-U

  6. Guevara P, Ramírez J L, Rojas E, Scorza JV, GonzálezN, Añez N. Leishmania braziliensis in blood 30years after cure. Lancet. 1993. 341:1341. https://doi.org/10.1016/0140-6736(93)90845-8

  7. Guevara P, Rojas E, Gonzalez N, Scorza JV, Añez N,Valera M, et al. Presence of Leishmania braziliensis inblood samples from cured patients or at different stagesof immunotherapy. Clin Diagn Lab Immunol. 1994.1:385-89. doi: 10.1128/cdli.1.4.385-389.1994.

  8. Añez N, Gonzalez C, Lugo de Yarbuh A, Rojas A,Crisante G, Ramírez JL. et al. Association of trauma withLeishmania parasite recall. Clinical and experimentalevidences. Bol Malariol Salud Ambient. 2000. 40(1-2):13-20.

  9. Corvalan FH, Sampaio RNR, Brustoloni YM, AndreottiR, Lima Júnior MSC. DNA identification of Leishmania(Viannia) braziliensis in human saliva from a patientwith American cutaneous leishmaniasis. J Venom AnimToxins Incl Trop Dis. 2011. 17(1): 98-102. doi:10.1590/S1678-91992011000100013.

  10. Phumee A, Kraivichian K, Chusri S, Noppakun N,Vibhagool A, Sanprasert V, et al. Detection of Leishmaniasiamensis DNA in saliva by polymerase chain reaction.Am J Trop Med Hyg. 2013. 89(5):899-905. doi: 10.4269/ajtmh.12-0612.

  11. Siriyasatien P, Chusri S, Kraivichian K, Jariyapan N,Hortiwakul T, Silpapojakul K, et al. Early detection ofnovel Leishmania species DNA in the saliva of two HIVinfectedpatients. BMC Infect Dis. 2016. 16:89. https://doi.org/10.1186/s12879-016-1433-2.

  12. Pandey N, Siripattanapipong S, Leelayoova S,Manomat J, Mungthin M, Tan-Ariya P, et al. Detectionof Leishmania DNA in saliva among patients withHIV/AIDS in Trang Province, southern Thailand.Acta Trop. 2018. Sep;185:294-300. doi: 10.1016/j.actatropica.2018.06.006.

  13. Brito MEF, Almeida EL, Medeiros ACR, Werkhäuser RP,Alexandre JLA, Sá BSLF, et al. Leishmania (Viannia)braziliensis isolated from the saliva of patients in acutaneous leishmaniasis-endemic area of northeasternBrazil. Mem Inst Oswaldo Cruz. 2018. Feb. 5;113(4):e170250. doi: 10.1590/0074-02760170250.

  14. Harris E, Kropp G, Belli A, Rodríguez B, Agabian N.Single-step multiplex PCR for characterization of NewWorld Leishmania complexes. J Clin Microbiol. 1998. 36(7):1989-1995. doi: 10.1128/jcm.36.7.1989-1995.1998

  15. Rojas A, Crisante G, García P, Carrero J, Añez N.Leishmania braziliensis-GPI anchored membraneproteins as an alternative tool for specific sero-diagnosisof active American cutaneous leishmaniasis. Am JMicrobiol Biotechnol. 2015. 2(4):57-63. http://article.aascit.org/file/pdf/9190765.pdf

  16. Premoli-de-Percoco G, Gonzalez N, Añez N, Guevara P,Ramírez JL. PCR detection of specific Leishmania-DNAin patients with periodontal disease. Pathologica. 2002.Feb; 94(1):28-31. PMID: 11912876.

  17. Añez N, Crisante G, Araujo S, Añez M, Rojas A, ParadaH. Detection and significance of Trypanosoma cruzipersistence in inflamed gingival foci in Chagas disease.Int J Clin Med Res. 2015a. 2(2):8-13. http://article.aascit.org/file/pdf/9060783.pdf

  18. Lee YH, Wong DT. Saliva: an emerging biofluid for earlydetection of diseases. Am J Dent. 2009. Aug;22(4):241-8. PMID: 19824562; PMCID: PMC2860957.

  19. Lima DP, Diniz DG, Moimaz SA, Sumida DH, OkamotoAC. Saliva: reflection of the body. Int J Infect Dis. 2010.Mar;14(3): e184-8. doi: 10.1016/j.ijid.2009.04.022.

  20. Javaid MA, Ahmed AS, Durand R, Tran SD. Saliva as adiagnostic tool for oral and systemic diseases. J Oral BiolCraniofac Res. 2016. Jan-Apr;6(1):66-75. doi: 10.1016/j.jobcr.2015.08.006. surgery. Subsequent studies shouldfocus on nutritional interventions that could shortenhospital length of stay.




2020     |     www.medigraphic.com