2021, Number 4
Antimicrobial activity of fractions obtained from essential oil of Minthostachys mollis against oral pathogens
Language: Spanish
References: 20
Page: 1-6
PDF size: 437.54 Kb.
ABSTRACT
Introduction: The essential oil of Minthostachys mollis has demonstrated to have important antimicrobial properties.Objective: To chemically characterize the fractions obtained from the essential oil of Minthostachys mollis and to evaluate the antimicrobial activity against Streptococcus mutans, Lactobacillus acidophilus, Enterococcus faecalis, Porphyromonas gingivalis and Candida albicans.
Material and Methods: The petroleum ether, dichloromethane and methanol fractions of the AE of M. mollis were chemically characterized by gas chromatography coupled to mass spectrometry. The repetitions of the antimicrobial test were calculated using the EPIDAT v.4.2 program. The antimicrobial activity was performed by the disk diffusion method and the minimum inhibitory concentration was calculated by the microdilution method. The data were analyzed using the ANOVA test (p=0.05).
Results: The main constituents of the petroleum ether, dichloromethane and methanol fractions were cis-Menthone (39,8 %), thymol (31,2 %) and α-Terpineol (43,6 %), respectively. All strains were sensitive to the three fractions, although C. albicans was the most sensitive strain, registering inhibition halos of 14,73±0.57 mm for the methanol fraction, 20,91±0.55 mm for petroleum ether and 20.38±0.58 mm for dichloromethane, finding significant differences when compared to 0,12 % Chlorhexidine and Nystatin (p<0,05). The minimum inhibitory concentrations of the fractions ranged from 0,2 to 3,2 μg/mL.
Conclusions: The main constituents of the petroleum ether, dichloromethane and methanol fractions were cis- Menthone, thymol and α-Terpineol. The petroleum ether and dichloromethane fractions were highly effective in inhibiting the growth of S. mutans, L. acidophilus, E. faecalis, P. gingivalis, and C. albicans.
REFERENCES
Valenzuela Oré F, Romaní Romaní F, Monteza Facho BM, Fuentes Delgado D, Vilchez Buitron E, Salaverry García O.Prácticas culturales vinculadas al cuidado de la salud y percepción sobre la atención en establecimientos de salud enresidentes de centros poblados alto-andinos de Huancavelica, Perú. Rev Peru Med Exp Salud Publica [Internet]. 2018[Citado 10/08/2020];35(1):84-92. Disponible en: https://doi:10.17843/rpmesp.2018.351.3603
De la Cruz MG, Malpartida SB, Santiafo HB, Jullian V, Bourdy G. Hot and cold: medicinal plant uses in Quechuaspeaking communities in the high Andes (Callejón de Huaylas, Ancash, Perú). J Ethnopharmacol [Internet]. 2014[Citado 10/09/2020];155(2):1093-117. Disponible en: https://doi:10.1016/j.jep.2014.06.042
Torrenegra Alarcón M, Granados Conde C, Durán Lengua M, León Méndez G, Yáñez Rueda X, Martínez C,et al. Composición química y actividad antibacteriana del aceite esencial de Minthostachys mollis. Orinoquia[Internet]. 2016 [Citado 10/09/2020];20(1):69-74. Disponible en: http://www.scielo.org.co/scielo.php?pid=S0121-37092016000100008&script=sci_abstract&tlng=es
Cano C, Bonilla P, Roque M, Ruiz J. In vitro antifungal activity and metabolites of the essential oil of the leavesof Minthostachys mollis (muña) leaves. Rev Peru Med Exp Salud Pública [Internet]. 2008 [Citado 10/09/2020];25(3):298-301. Disponible en: http://www.scielo.org.pe/scielo.php?pid=S1726-46342008000300008&script=sci_abstract&tlng=en
Campo FM, Ambuludí FDL, Cepeda RNC, Márquez HI, San Martín GD, Cuesta RO. Composición química y actividadantibacteriana del aceite esencial de Minthostachys mollis Griseb contra el Staphylococcus aureus. Rev Cubana Farm[Internet]. 2019 [Citado 10/09/2020];51(4):[Aprox. 2 p.]. Disponible en: http://www.revfarmacia.sld.cu/index.php/far/article/view/183/175
Peña SD, Gutiérrez RM. Efecto antimicrobiano del aceite esencial de Minthostachys mollis sobre microorganismosfrecuentes en vías respiratorias bajas. Revista Ciencia y Tecnología [Internet]. 2017 [Citado 10/09/2020];13(3):55-66.Disponible en: https://revistas.unitru.edu.pe/index.php/PGM/article/view/1874/1802
Benites J, Guerrero Castilla A, Salas F, Martinez JL, Jara Aguilar R, Venegas Casanova EA, et al. Chemicalcomposition, in vitro cytotoxic and antioxidant activities of the essential oil Peruvian Minthostachys mollis Griseb.Bol Latinoam Caribe Plant Med Aromat [Internet]. 2018 [Citado 10/09/2020];17:566-74. Disponible en: https://revistaschilenas.uchile.cl/handle/2250/34890
Díaz LK, Moromi NH. Determinación antibacteriana in vitro de Menthostachys mollis (Muña) frente a bacterias oralesde importancia estomatológica. Odonto Sanmarquina [Internet]. 2005 [Citado 10/09/2020];8(2):3-5. Disponible en: https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/3137
Aguilar Ancori EG, Aguilar Ancori KV, Garay B, Mamani V, Quispe Flórez MM. Actividad antibacteriana frente aStreptococcus mutans de aceites esenciales de cinco plantas alto andinas. Rev Peru Med Exp Salud Publica [Internet].2018 [Citado 10/09/2020];35(1):161-3. Disponible en: https://doi:10.17843/rpmesp.2018.351.3610
Hendry ER, Worthington T, Conway BR, Lambert PA. Antimicrobial efficacy of eucalyptus oil and 1,8-cineolealone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilmcultures. J Antimicrob Chemother [Internet]. 2009 [Citado 10/09/2020];64(6):1219-25. Disponible en: https://doi.org/10.1093/jac/dkp362