medigraphic.com

2023, Number 3

<< Back Next >>

Enf Infec Microbiol 2023; 43 (3)

Epstein-Barr virus, cytomegalovirus and human papilloma virus infection in women from Chiapas, Mexico

Sánchez GRA, Domínguez AS, Reyes TMJ, Sepúlveda DJ, Ordóñez GJA, Domínguez ES, Trujillo VMG

Language: Spanish
References: 14
Page: 87-91
PDF size: 211.32 Kb.


ABSTRACT

Objective. To determine the frequency of infection of Epstein-Barr virus (EBV), cytomegalovirus (HCMV) and human papillomavirus (HPV) through the RT-PCR technique in cervical samples from patients treated in a tertiary care hospital in Chiapas.
Materials and methods. A descriptive, cross-sectional and prospective study was developed. Through convenience non-probability sampling, a total of 104 samples were collected, 89 of which met the inclusion criteria. A questionnaire with variables of interest was used, an informed consent was obtained. To each patient who accepted, a cervical sample was taken by a gynecologist. The statistical analysis applied was descriptive. dna extraction was by commercial kit, quality was verified by endpoint PCR and agarose gel electrophoresis; the detection of the viruses was by real-time PCR (RT-PCR), using specific assays for each virus with the respective reaction controls.
Results. The mean age was 47.18 (±12.04) years, 48.3% (n = 43) were married, 78% (n = 69) were found to be positive for the presence of viral DNA; the highest frequency was due to HPV with 46.1% (n = 41). HPV coinfection with HCMV and/or EBV was found in 27% (n = 24).
Conclusions. Viral infections play an important role in the genesis of cervical cancer, several studies have found genomic integration of viruses such as: HPV, EBV, among others in the cells of the cervix, causing the triggering of oncogenic processes. Identifying the infection with these viruses provides guidelines for the management and monitoring of the patient with lesions in the cervix.


REFERENCES

  1. Buskwofie, A., David-West, G. y Clare, C.A., “A reviewof cervical cancer: incidence and disparities”, J NatlMed Assoc, 2020, 112 (2): 229-232. doi: 10.1016/j.jnma.2020.03.002. Epub, 8 de abril de 2020. pmid:32278478. Disponible en: https://pubmed.ncbi.nlm.nih.gov/32278478/.

  2. “Cáncer cervicouterino”, s/f. Who.int. Recuperado el 29de noviembre de 2022. Disponible en: https://www.who.int/es/news-room/fact-sheets/detail/cervical-cancer.

  3. Olusola, P., Banerjee, H.N., Philley, J.V. y Dasgupta,S., “Human papilloma virus-associated cervical cancerand health disparities”, Cells, 2019, 8 (6): 622.doi: 10.3390/cells8060622. pmid: 31234354; pmcid:pmc6628030. Disponible en: https://pubmed.ncbi.nlm.nih.gov/31234354/.

  4. “Cancer of the cervix uteri-cancer stat facts”, seer. Citadoel 6 de octubre de 2022. Disponible en: https://seer.cancer.gov/statfacts/html/cervix.html.

  5. Bhatla, N. y Singhal, S., “Primary hpv screening for cervicalcancer”, Best Pract Res Clin Obstet Gynaecol, 2020, 65: 98-108. doi: 10.1016/j.bpobgyn.2020.02.008. Epub:2 de marzo de 2020. pmid: 32291178. Disponible en: https://pubmed.ncbi.nlm.nih.gov/32291178/.

  6. Staykova, J., Belovska, T., Murad, A., Kakid, S., Nacheva,A. y Shikova, E., “Cervical viral infections among asymptomaticBulgarian women”, Cent Eur J Public Health,2016, 24 (3): 176-179. doi: 10.21101/cejph.a4299. pmid:27760284.

  7. Adeyemi, O.O., Herod, M.R., Oladiji, F., Fakunle, Y.M.,Babatunde, A.S. y Agbede, O.O., “A multi-template multiplexpcr assay for hepatitis b virus and human β-globin”,J Med Virol [Internet], 2017, 89 (11): 1944-1951.

  8. Draganov, P., Todorov, S., Todorov, I., Karchev, T. yKalvatchev, Z., “Identification of hpv dna in patients withjuvenile-onset recurrent respiratory papillomatosis usingsybr Green real-time pcr”, Int J Pediatr Otorhinolaryngol,2006, 70 (3): 469-473. doi: 10.1016/j.ijporl.2005.07.018.Epub: 30 de Agosto de 2005. pmid: 16137771. Disponibleen https://pubmed.ncbi.nlm.nih.gov/16137771/.

  9. Khoddami, M., Nadji, S.A., Dehghanian, P., Vahdatinia,M. y Shamshiri, A.R., “Detection of Epstein-Barr virus dnain Langerhans cell yistiocytosis”, Jundishapur J Microbiol,2015, 8 (12): e27219. doi: 10.5812/jjm.27219. pmid:26870310; pmcid: pmc4746794. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746794/.

  10. Gómez-Mora, E., Massanella, M., García, E., Giles, D.,Bernadó, M., Urrea, V., Carrillo, J., Ouchi, D., Puig, J.,Negredo, E., Clotet, B., Blanco, J. y Cabrera, C., “Elevatedhumoral response to cytomegalovirus in hiv-infectedindividuals with poor cd4+ t-cell immune recovery”,plos One, 2017, 12 (9):e0184433. doi: 10.1371/journal.pone.0184433. pmid: 28934217; pmcid: pmc5608209. Disponibleen: https://pubmed.ncbi.nlm.nih.gov/28934217/.

  11. Naushad, W., Surriya, O. y Sadia, H., “Prevalence ofebv, hpv and mmtv in Pakistani breast cancer patients:a possible etiological role of viruses in breast cancer”,Infect Genet Evol, 2017, 54: 230-237. doi: 10.1016/j.meegid.2017.07.010. Epub: 10 de julio de 2017. pmid:28705719. Disponible en: https://pubmed.ncbi.nlm.nih.gov/28705719/.

  12. Putri, N.D., Wiyatno, A., Dhenni, R., Sriyani, I.Y., Dewantari,A.K., Handryastuti, S. et al., “Birth prevalence andcharacteristics of congenital cytomegalovirus infectionin an urban birth cohort, Jakarta, Indonesia”, Int J InfectDis, 2019, 86: 31-39. Disponible en: https://pubmed.ncbi.nlm.nih.gov/31207385/.

  13. Golrokh Mofrad, M., Kazeminezhad, B. y Faghihloo, E.,“Prevalence of Epstein-Barr virus (ebv) in Iranian breastcarcinoma patients”, Asian Pac J Cancer Prev, 2020, 21(1): 133-137. doi: 10.31557/APJCP.2020.21.1.133. pmid:31983175; pmcid: pmc7294004. Disponible en: https://pubmed.ncbi.nlm.nih.gov/31983175/.

  14. Naqvi, S.U., Khan, S., Ahmed, A., Lail, A., Gul, S. yAhmed, S., “Prevalence of ebv, cmv, and hpv in oral squamouscell carcinoma patients in the Pakistani population”,J Med Virol, 2020. doi: 10.1002/jmv.25791. Epubdisponible. pmid: 32219873. Disponible en: https://pubmed.ncbi.nlm.nih.gov/32219873/.




2020     |     www.medigraphic.com