medigraphic.com
ISSN 1561-2988 (Print)

2021, Number 3

<< Back Next >>

Rev Cubana Farm 2021; 54 (3)

Evaluation of the performance of analytical methods for extracellular redox state in human serum

Cruz VH, Cala CL, Ramos HL, Roque RC

Language: Spanish
References: 18
Page: 1-14
PDF size: 960.29 Kb.


ABSTRACT

Introduction:v The evaluation of redox state in biological fluids is of great importance in the field of medicine, if it is considered the demonstrated relationship of oxidative stress with multiple pathophysiological processes. There are methods to assess extracellular redox state, but compliance with their quality criteria has not been shown in the Cuban context.
Objective: Assess the performance of the analytical methods used as a measure of extracellular redox status in human serum for efficient use in future studies.
Methods: Based on a cross-sectional analytical study conducted at the Basic Sciences Laboratory of the University of Medical Sciences of Santiago de Cuba, the methods used for the evaluation of extracellular redox state in blood serum were validated through values of accuracy, specificity, linearity and precision (repeatability and intermediate precision).
Results: The assessment of the different analytical methods showed their high specificity, and also quantifiable values only in samples corresponding to the evaluated analytes. The calibration curves for malonldialdehyde and reduced glutathione were found to be linear with correlation coefficients ≥ 0.995. The variation coefficients of the repeatability studies were under the 3%, while in the intermediate accuracy analyses the ANOVA indicated non-significant differences between the measurements.
Conclusions: The standardization of the analytical methods used as a measure of the extracellular redox state in human serum from the use of quality evaluation parameters demonstrate, by the characteristics of their performance, that they meet the requirements for their application in medical practice and biomedical research.


REFERENCES

  1. Carvajal Carvajal C. Especies reactivas del oxígeno: formación, función y estrésoxidativo. Med leg. Costa Rica. 2019 [acceso 04/06/2021];36(1):91-100. Disponibleen: http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S1409-00152019000100091&lng=en

  2. González del Pozo I. Biomarcadores de estrés oxidativo y capacidadantioxidante en el paciente con hipertrofia cardiaca: estudio observacional. [Tesisdoctoral, Programa de Doctorado en Investigación en Ciencias Médico-Quirúrgicas]. [Madrid]: Universidad Complutense; 2019 [acceso 05/06/2021].Disponible en: https://eprints.ucm.es/id/eprint/57665/1/T41428.pdf

  3. Mañon Rossi W, Garrido G, Núñez Sellez AJ. Biomarcadores del estrés oxidativoen la terapia antioxidante. Journ of Pharm & Pharmacog Res. 2016;4(2):62-83Disponible en: http://www.redalyc.org/articulo.oa?id=496053934003

  4. Arteche Hidalgo L, García Sánchez M, Leyva Cerulia M, Martínez Martín S.Estandarización de valores de referencia de parámetros de estrés oxidativo. RevCub Med Mil. 2018 [acceso 05/06/2021];47(2):127-32. Disponible en:http://www.revmedmilitar.sld.cu/index.php/mil/article/view/102/169

  5. Iraizoz Barrios AM, Crespo Ferrá Y, Delgado Roche L, León Fernández OS, FloresSánchez RM, Brito Sosa G, et al. Influencia del estrés oxidativo seminal en elresultado de técnicas de fertilización in vitro. Rev Cumbres. 2017 [acceso05/06/2021];3(2):31-40. Disponible en:https://investigacion.utmachala.edu.ec/revistas/index.php/Cumbres/article/view/124

  6. Forbes-Hernández TY, Betancourt G, Rodríguez D, García MA. Capacidadantioxidante total de la dieta vs. balance redox. QhaliKay. Rev Cien Salud. 2020[acceso 05/06/2021];4(1):35-4. Disponible en:https://revistas.utm.edu.ec/index.php/QhaliKay/article/view/2711/2943

  7. Centro para el Control Estatal de Medicamentos, Equipos y Dispositivos Médicos(CECMED). Anexo No. I. De las buenas prácticas para laboratorio de control demedicamentos. Validación de métodos analíticos. La Habana: Minsap; 2013.[acceso 05/06/2021]. Disponible en:https://www.cecmed.cu/sites/default/files/adjuntos/Reglamentacion/validacion_de_metodos_analiticos.pdf

  8. Ministerio de Salud. Guía de Validación de Métodos Analíticos. Cotra Rica:Ministerio de Salud; 2019 [acceso 05/06/2021]. Disponible en:https://bit.ly/2TIT0gP

  9. Mederos-Pérez I, Vázquez-Silva Y, De la Cruz-Fernández CY, López-Lamezón S.Efecto del tratamiento farmacológico de la diabetes sobre parámetros bioquímicosclásicos y estado redox. Rev Mex Patol Clin Med Lab. 2020 [acceso05/06/2021];67(1):17-25. Disponible en:https://www.medigraphic.com/pdfs/COMPLETOS/patol/2020/pt201.pdf

  10. Gil L, Daumy Y, Pérez L, Alvarez A, Díaz H, Luzardo C, et al. Evaluación delestado redox y de algunos marcadores inmunológicos en individuos seropositivos alvirus linfo-trópico de células T humanas tipo I. Bioq. 2002;27(2): 6-31

  11. Esterbauer H, Schaur R, Zollner H. Chemistry and Biochernistry of 4-Hydroxynonenal, Malondialdehyde and Related Aldehydes. Free Rad. Bio! & Med.1991;11:81-128.

  12. Aebi H. Catalase in vitro. Meth Enzymol. 1984;105:121-6.

  13. Sedlak J, Lindsay RH. Estimation of total protein bound and non-proteinsulfhydryl group in tissue with Ellman´s reagent. Anal Biochem.1968;25:192-205.

  14. Castro EM. Bioestadística aplicada en investigación clínica: conceptos básicos.Rev méd clín los condes. 2019 [acceso 05/06/2021];30(1):50-65. Disponible en:https://www.sciencedirect.com/science/article/pii/S0716864019300045

  15. Manterola C, Quiroz G, Salazar P, García N. Methodology of study designs mostfrequently used in clinical research. Rev méd clín los condes. 2019 [acceso05/06/2021];30(1):36-49. Disponible en:https://www.sciencedirect.com/science/article/pii/S0716864019300057

  16. Cruz Vadell H, León Nip M, Cáceres Diéguez A, López Barroso R, ÁlvarezGuerra ED. El análisis multivariado a partir del estado redox asociado a lapreeclampsia. Rev Cubana Obstet Ginecol. 2017 [acceso 05/06/2021];43(3):107-

  17. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0138-600X2017000300010&lng=es.

  18. Isaza Martínez JH, Restrepo Vega MJ, Colmenares Dulcey AJ. N-methyl-2-phenylindole colorimetric method as biomarker for lipid peroxidation in Pisumsativum. International Journal of Phytocosmetics and Natural Ingredients.2015;2:10. DOI: 10.15171/ijpni.2015.10




2020     |     www.medigraphic.com