medigraphic.com
ISSN 1561-2988 (Print)

2021, Number 4

<< Back Next >>

Rev Cubana Farm 2021; 54 (4)

Antibacterial activity of essential oils of plants growing in Colombia and its effect on the activity of β-lactam antibiotics

Barrios MSX, Stashenko EE, Ocazionez JRE, Fuentes LJL

Language: Spanish
References: 24
Page: 1-15
PDF size: 706.87 Kb.


ABSTRACT

Introduction: Methicillin-resistant Staphylococcus aureus is a serious public health problem and the most common cause of hospital infection worldwide.
Objective: Assess the antibacterial activity against methicillin-resistant Staphylococcus aureus of essential oils from plants growing in Colombia and its potentiating effects on the activity of β-lactam antibiotics.
Methods: The antibacterial activity of twenty-seven essential oils obtained by microwave-assisted hydrodistilation was assessed, for which the micro-dilution method was used, both in Staphylococcus aureus strains resistant to methicillin and in Staphylococcus aureus sensitive to methicillin. To this end, their minimum inhibitory concentration values were determined. The enhancing effect of essential oils on the activity of β-lactam antibiotics was evaluated using cotreatment procedures in the methicillin-resistant strain of Staphylococcus aureus.
Results: Based on the values of minimum inhibitory concentration in methicillinresistant Staphylococcus aureus, the essential oils of Psidium sartorianum and Turnera diffusa showed antibacterial effect, and at non-inhibitory concentrations, significantly reduced the values of minimum inhibitory concentration of antibiotics in the Staphylococcus aureus strain resistant to methicillin.
Conclusions: The results indicate that the essential oils of P. sartorianum and T. diffusa have antibacterial properties and may enhance the activity of β-lactam antibiotics in methicillin-resistant Staphylococcus aureus. The species P. sartorianum and T. diffusa are sources of agents that modify bacterial resistance to antibiotics.


REFERENCES

  1. Diekema DJ, Pfaller MA, Schmitz FJ, Smayevsky J, Bell J, Jones RN, et al.Survey of infections due to Staphylococcus species: Frequency of occurrence andantimicrobial susceptibility of isolates collected in the United States, Canada,Latin America, Europe, and the Western Pacific Region for the SENTRYantimicrobial surveillance program, 1997–1999. Clin. Infect. Dis. 2001;32(Suppl 2):S114-S132. DOI: 10.1086/320184

  2. Enright MC, Robinson DA, Randle G, Feil EJ, Grundmann H, Spratt BG. Theevolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc.Natl. Acad. Sci. U.S.A. 2002;99(11):7687-92. DOI: 10.1073/pnas.122108599

  3. Cong Y, Yang S, Rao X. Vancomycin resistant Staphylococcus aureusinfections : A review of case updating and clinical features. J. Adv. Res.2020;21(January):169-76. DOI: 10.1016/j.jare.2019.10.005

  4. Rowland SJ, Dyke KGH. Tn552, a novel transposable element fromStaphylococcus aureus. Mol. Microbiol. 1990;4:961-75. DOI: 10.1111/j.1365-2958.1990.tb00669.x

  5. Haaber J, Penadés JR, Ingmer H. Transfer of Antibiotic Resistance inStaphylococcus aureus. Trends Microbiol. 2017;25(11):893-905. DOI:10.1016/j.tim.2017.05.011

  6. Maiques E, Úbeda C, Campoy S, Salvador N, Lasa Í, Novick RP, et al. β-Lactamantibiotics induce the SOS response and horizontal transfer of virulence factors inStaphylococcus aureus. J. Bacteriol. 2006;188(7):2726-9. DOI:10.1128/jb.188.7.2726-2729.2006

  7. Podlesek Z, Žgur Bertok D. The DNA Damage Inducible SOS Response Is a KeyPlayer in the Generation of Bacterial Persister Cells and Population WideTolerance. Front. Microbiol. 2020;11(August):1785. DOI:10.3389/fmicb.2020.01785

  8. Abreu AC, McBain AJ, Simões M. Plants as sources of new antimicrobials andresistance-modifying agents. Nat. Prod. Rep. 2012;29(9):1007-21. DOI:10.1039/c2np20035j

  9. Lahmar A, Bedoui A, Mokdad-Bzeouich I, Dhaouifi Z, Kalboussi Z, Cheraif I, etal. Reversal of resistance in bacteria underlies synergistic effect of essential oilswith conventional antibiotics. Microb. Pathog. 2017;106(May):50-9. DOI: 10.1016/j.micpath.2016.10.018

  10. Chaves TP, Pinheiro REE, Melo ES, Soares MJ dos S, Souza JSN, Andrade TBde, et al. Essential oil of Eucalyptus camaldulensis Dehn potentiates β-lactamactivity against Staphylococcus aureus and Escherichia coli resistant strains. Ind.Crops Prod. 2018;112 (February):70-4. DOI: 10.1016/j.indcrop.2017.10.048

  11. Jugreet BS, Mahomoodally MF. Essential oils from 9 exotic and endemicmedicinal plants from Mauritius shows in vitro antibacterial and antibioticpotentiating activities. South African J Bot. 2020;132(August):355-62. DOI:10.1016/j.sajb.2020.05.001

  12. Sibandze GF, Stapleton P, Gibbons S. Constituents of two Dioscorea speciesthat potentiate antibiotic activity against MRSA. J Nat Prod. 2020;83(5):1696-1700. DOI: 10.1021/acs.jnatprod.9b01006

  13. Seebaluck-Sandoram R, Lall N, Fibrich B, van Staden AB, Mahomoodally F.Antibiotic-potentiating activity, phytochemical profile, and cytotoxicity ofAcalypha integrifolia Willd. (Euphorbiaceae). J. Herb Med. 2018;11(March):53-9.DOI: 10.1016/j.hermed.2017.03.005

  14. Eloff JN. A sensitive and quick microplate method to determine the minimalinhibitory concentration of plant extracts for bacteria. Planta Med.1998;64(8):711-13. DOI: https://doi.org/10.1055/s-2006-957563

  15. Cos P, Vlietinck AJ, Berghe DV, Maes L. Anti-infective potential of naturalproducts: how to develop a stronger in vitro “Proof-of-Concept”. J.Ethnopharmacol. 2006;106(3):290-302. DOI: 10.1016/j.jep.2006.04.003

  16. R Core Team. R: A Language and environment for statistical computing.Vienna, Austria: R foundation for statistical computing; 2013 [acceso28/09/2021]. Disponible en. https://www.r-project.org

  17. Morais-Braga MFB, Sales DL, Carneiro JNP, Machado AJT, dos Santos ATL, deFreitas MA, et al. Psidium guajava L. and Psidium brownianum Mart ex DC:Chemical composition and anti-Candida effect in association with fluconazole.Microb. Pathog. 2016;95:200-07. DOI: 10.1016/j.micpath.2016.04.013

  18. Morais-Braga MFB, Carneiro JNP, Machado AJT, dos Santos ATL, Sales DL,Lima LF, et al. Psidium guajava L., from ethnobiology to scientific evaluation:Elucidating bioactivity against pathogenic microorganisms. J. Ethnopharmacol.2016;194:1140-52. DOI: 10.1016/j.jep.2016.11.017

  19. Bueno J, Escobar P, Martínez JR, Leal SM, Stashenko EE. Composition of threeessential oils, and their mammalian cell toxicity and antimycobacterial activityagainst drug resistant-tuberculosis and nontuberculous mycobacteria strains. Nat.Prod. Commun. 2011;6:1743–1448. DOI: 10.1177/1934578X1100601143

  20. Szewczyk K, Zidorn C. Ethnobotany, phytochemistry, and bioactivity of thegenus Turnera (Passifloraceae) with a focus on damiana–Turnera diffusa. J.Ethnopharmacol. 2014;152:424-43. DOI: 10.1016/j.jep.2014.01.019

  21. Burt S. Essential oils: their antibacterial properties and potential applicationsin foods–a review. Int. J. Food Microbiol. 2004;94:223-53. DOI:10.1016/j.ijfoodmicro.2004.03.022

  22. Pino JA, Bello A, Urquiola A, Aguaro J, Marbot R. Leaf oils of Psidiumcymosum Urb. and Psidium sartorianum Niedz. from Cuba. J. Essent. Oil Res.2003;15:184-8. DOI: 10.1080/10412905.2003.9712107

  23. Stashenko E, Martínez JR. The expression of biodiversity in the secondarymetabolites of aromatic plants and flowers growing in Colombia. In: Potential ofessential oils, Chapter 4, IntechOpen Publisher, London, UK, 2018:59-86. DOI:10.5772/intechopen.78001

  24. Coutinho HDM, Costa JGM, Lima EO, Falcão-Silva VS, Siqueira-Júnior JP.Herbal therapy associated with antibiotic therapy: potentiation of the antibioticactivity against methicillin– resistant Staphylococcus aureus by Turnera ulmifoliaL. BMC Compl. Alternative Med. 2009;9:13. DOI: 10.1186/1472-6882-9-13




2020     |     www.medigraphic.com