Valdés FY, Concepción ÁA, Acosta ST, Correa JSB

Language: Spanish
References: 17
Page: 1-20
PDF size: 680.31 Kb.

ABSTRACT

Introduction: The National Center for Medical Genetics implemented the external quality control of hemoglobin electrophoresis in the hemoglobinopathy program in the National Genetics Network in 2009. The implementation of this control leads to evaluating the analytical accuracy of the laboratory, comparing results obtained from the same sample between the reference laboratory and the provincial laboratories.
Objective: To describe the results of the external quality control of hemoglobin electrophoresis and the frequency of genotypic variants in the hemoglobinopathy program, period 2009 - 2019.
Methods: A descriptive and cross-sectional observational study was carried out, using statistical data from the Quality Control Registry. Random samples (5 and 15) of blood with EDTA were processed monthly by province. The HYDRASYS 2 equipment was used in the analysis of hemoglobin variants and thalassemias. The evaluation of the program was performed: rejected samples (pre-analytical phase), processed and positive samples (analytical phase), discrepant samples in the results (post-analytical phase).
Results: 10 370 controls were received, 10 090 were processed and 2.8% of the samples were rejected. In 3477 controls, a variation in the structure, quantity and / or function of the hemoglobin molecule was observed. 1.2% of the analyzed samples presented discrepancies in the results.
Conclusions: The electrophoresis EQC showed satisfactory results, despite the fact that some evaluated laboratories do not comply with all the procedures and criteria of the phases evaluated in the diagnosis of hemoglobinopathy. It is confirmed that the most frequent genotypic variant in Cuba is AS hemoglobinopathy.

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