Pastrana-Pineda C, Gonce J, López N, Saavedra E, Belmont-Díaz JA

Language: Spanish
References: 0
Page: 42-47
PDF size: 836.79 Kb.

ABSTRACT

Oxidative phosphorylation (OxPhos) is the culmination of catabolism in aerobic organisms. This process is responsible for generating the most of ATP molecules that an organism needs to live. Despite the importance that OxPhos plays in the cell, we rarely know the rate that this process must have for the cell to carry out its vital processes. In this work, we analyze the OxPhos flow with a high-resolution oxygraph (Oroboros Insturment^®) to calculate the rate of the production of ATP using Trypanosoma cruzi as a model. The fact that this parasite has only one mitochondrion offers a unique opportunity to study a fully intact individual mitochondrion.