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Anales de Otorrinolaringología Mexicana

2024, Number 3

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Otorrinolaringología 2024; 69 (3)

Effect of brain derived neurotrophic factor on the regeneration of facial nerve

Cristerna SL, Brulé ACD, Olmos ZJR, Silva MM, Gomes-da Silva RP, Gaxiola GM, Romero RLP

Language: Spanish
References: 21
Page: 163-174
PDF size: 857.82 Kb.


ABSTRACT

Objective: To evaluate the clinical, macroscopic and histological changes and in the expression of myelin basic protein and glial fibrillary acidic protein postneurorraphy of the facial nerve combined with the application of brain derived neurotrophic factor or placement of a sural nerve graft in guinea pigs.
Materials and Methods: An experimental, prospective, longitudinal and comparative study was done at Unit of Experimental Lung Transplant of National Institute of Respiratory Diseases Ismael Cosio Villegas, Mexico City. In 18 guinea pigs, divided into 3 groups of 6 each one, neurorraphy of the left facial nerve was performed and they were treated as follows: Group I: Facial nerve neurorraphy without treatment. Group II: sural nerve graft placement without treatment. Group III: Facial nerve neurorraphy and brain derived neurotrophic factor. The study lasted 12 weeks and eyelid closure, vibrissa movements, infection, necrosis, adhesions and fibrosis of the anastomosis were evaluated. Study was done from January 1st, 2022 to January 31, 2023.
Results: Group III showed better clinical evolution in a shorter time, less macroscopic and microscopic fibrosis, better axonal regeneration and neovascularization. The 3 groups showed similar expression of myelin basic protein and glial fibrillary acidic protein.
Conclusions: Facial nerve neurorraphy in guinea pigs combined with the application of brain derived neurotrophic factor reduces the degree of facial paralysis in a shorter time and favors the healing of the neurorraphy with less fibrosis and better axonal regeneration than the neurorraphy without treatment and sural graft placement without treatment, but it does not originate changes in the production of basic protein and glial fibrillary acidic protein.


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