Rojas BMM, Heydrich PM, Sánchez CD, Tejera HB, Ríos RY, Lugo MD

Language: Spanish
References: 9
Page: 25-11
PDF size: 337.18 Kb.

ABSTRACT

The use of Plant Growth-Promoting Bacteria (PGPB) allows for diminishing the use of chemical products in agriculture. These products are dangerous for environments and human health. The objective of the present work was to characterize isolates from Bacilli class to select potentially efficient strains for their use in sustainable agriculture. To isolate bacteria; the samples from rhizosphere and rice, maize and coffee plants were heated to 80 ºC. The isolates were characterized according to PGPB and antagonistic traits. Several strains were selected to study the colonization, diversity and their effect on plant growth. One hundred forty-five aerobic Gram-positive endospore forming bacteria were isolated from rice, maize and coffee. The studied strains showed several traits for plant growth. In thirty-seven selected strains from rice, maize and coffee, antagonistic activity was demonstrated against phytopathogenic fungi. After that, two strains were selected and they show their ability to colonize rice plants, move and form biofilms. The promised strains were identified as belonging to Bacillus genus by sequencing the 16S rRNA gene. The molecular study of Bacillus from rice demonstrated the high genetic diversity and the occurrence of virulence determinants in few strains. The inoculation of maize and tomato in semicontrolled conditions showed positive effects in plant growth. An integrally characterized bacterial native collection, efficient for plant growth promotion and antagonistic activity is available, which constitutes the base for new bioproducts obtainment. This work proposes an efficient methodology for prospecting and characterizing Bacillus for their use in agricultural sustainability. This work received the Annual Award of the Cuban Academy of Sciences for the year 2020.

REFERENCES

1. Rojas MM, Tejera B, Bosch DM, Ríos Y, Rodríguez J, Heydrich M. Potencialidades de cepas de Bacillus para la promoción del crecimiento en el cultivo del Zea mays L. Cuban J Agric Sci. 2016;50(3):485-96.

2. Rojas MM, Hernández A, Rives N, TejeraB, Acebo Y, Heydrich M. Producción deun antisuero para la detección de ácidoindoalcético en cultivos de bacterias promotorasdel crecimiento vegetal. Acta BiolColombiana. 2012;17(2):271-80.

3. Sánchez D, González L, del Monte-MartínezA, Rojas MM. Factibilidad biológicadel uso de cepas de Bacilli productoras deácido 3 indolacético en el crecimiento invitro del cultivo del arroz. Rev Cub CiencBiol. 2019;7(1):1-10.

4. Rojas MM, Rodríguez AJ, González L,Heydrich M. Influencia de diferentes factoresen el crecimiento de bacterias endófitasde caña de azúcar. Rev ColombianaBiotecnol. 2015;XVII(2):149-55.

5. Rojas MM, Tejera B, Larrea JA, MahillonJ, Heydrich M. Aislamiento y caracterizaciónde cepas de Bacillus asociadas alcultivo del arroz (Oryza sativa L.). Rev BrasAgroecol. 2011;6(1):90-9.

6. Rojas MM, Sánchez D, Rosales K, Lugo D.Antagonismo de Bacillus frente a hongosfitopatógenos de cultivos hortícolas. RevProtección Veg. 2017;32(2):1-9.

7. Rojas MM, Bello MA, Ríos Y, Lugo D,Rodríguez J. Estimulación del crecimientode cultivos de interés económico por cepasde Bacillus. Acta Agron. 2020;69(1):54-60.

8. Tejera B, Heydrich M, Rojas MM. Aislamientode Bacillus solubilizadores defosfatos asociados al cultivo del arroz.Agron Mesoam. 2013;24(2):357-64.

9. Tejera B, Heydrich M, Rojas MM. Antagonismode Bacillus sp. contra hongosfitopatógenos del cultivo del arroz (Oryzasativa L.). Rev Protección Veg. 2012;27(2):117-22.