Padilla-Garfias F, Araiza-Villanueva MG, Peña DA

Language: Spanish
References: 123
Page: 213-234
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ABSTRACT

Molecular techniques for gene expression analysis have significantly evolved over time and are now fundamental to understanding gene regulation in diverse organisms, including fungi. From the classical techniques such as Northern Blot and RNase Protection Assay to more modern tools such as Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) and Next Generation Sequencing (NGS) of ribonucleic acid (RNA), these methodologies have revolved genetic research. These techniques enable precise study of gene expression levels and variability, facilitating identi-fication of key genes and revealing patterns of gene expression and regulation. With increasingly sophisticated applications, these approaches provide detailed insight into gene dynamics, significantly contributing to the advancement of knowledge in molecular biology and genetics. In this review we provide a historical overview of the main techniques used to study gene expression, with an emphasis on studies conducted in fungi, with the aim to provide a perspective on the utility and importance of these methodologies.

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