Gómez GJ, García ÁR, Chávez PJL, Rivera EL, Alemón MR

Language: Spanish
References: 24
Page: 3-12
PDF size: 242.46 Kb.

ABSTRACT

Introductioón: Quercetin (QT) is a plant flavonoid with antioxidant and antidiabetic properties that could improve the treatment of children and adolescents with obesity and diabetes. It has recently been marketed as food supplements; however, the toxicity and safe doses of QT in humans have not been concluded.
Aim To determine the toxicity of QT in cultured healthy human cells and assess its effect on extracellular glucose movilization.
Materials and Methods: HA19 cells were grown to confluence in DMEM-F12 medium supplemented with 10% FBS, 1% antibiotic and antifungal, at 37^oC with 5% CO2 atmosphere. They were exposed to QT concentrations of 0.3 to 30 µg/mL for 24 hours. Three presentations of quercetin were used: pure standard (QTS), food supplement (QTC) and aqueous extract of moringa (Mor). QT toxicity was assessed by staining of surviving cells with crystal violet, and extracellular glucose concentration by glucose oxidase activity.
Results: QTS caused cell death proportional to the increase in its concentration. QTC was more toxic at low concentrations. The QT in the plant extract (Mor) had a cytoprotective effect at the highest concentrations. QT induced glucose transport from the medium to the cells in all three presentations.
Conclusions: QT in commercial form (QTC) was more cytotoxic than the plant extract, so the flavonoid from a natural source could be safer to consume.

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