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2025, Number 1

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Enf Infec Microbiol 2025; 45 (1)

Molecular detection of sexually transmitted pathogens at Instituto Nacional de Perinatología

López HM, Guerra IFM, Cerano FJL

Language: Spanish
References: 40
Page: 7-17
PDF size: 380.69 Kb.


ABSTRACT

Background: sexually transmitted infections (stis) are a global reproductive health problem. The development of new molecular methods has led to the implementation of techniques that can detect several sti-causing microorganisms in the same sample.
Objective: to identify 12 sexually transmitted pathogens in endocervical samples from women attending in a third-level institution.
Material and methods: a commercial kit was used for identifying 11 pathogens and an endpoint pcr were used to identify Gardnerella vaginalis.
Results: Ureaplasma spp. was identified in 88.5% (262/296) of the samples, Mycoplasma hominis in 27.7% (82/296), Mycoplasma genitalium in 7.1% (21/296), Chlamydia trachomatis in 9.5% (28/296), Neisseria gonorrhoeae in 0.33% (1/296), G. vaginalis in 10.5% (31/296) and Trichomonas vaginalis in 17.9% (53/296).
Conclusion: pregnant women showed a higher frequency of T. vaginalis infection (OR 2.65; 95% CI 1.4-5.0; p ‹0.05) and a significant association with the presence of M. hominis in women aged 20 to 24 years (OR 3.3; 95% CI 1.29- 8.3; p ‹0.02). In infertile women aged 30 to 34 years, there was an association between C. trachomatis infection (OR 3.6; 95% CI 1.03-12.6) and GV (OR 2.82; 95% CI 1.07-7.44; p ‹0.05). This suggests that routine diagnosis of these pathogens should continue, as they can develop various gynecological and obstetric pathologies.


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