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ISSN 1028-9933 (Electronic)

2022, Number 3

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RIC 2022; 101 (3)

In vivo pyrogenicity evaluation of fungal bioproducts with prebiotic potential

Lebeque-Pérez Y, Fong-Lores O, Rodríguez-Leblanch E, Llauradó-Maury G, Serrat-Díaz MJ

Language: Spanish
References: 16
Page: 1-12
PDF size: 824.91 Kb.


ABSTRACT

Introduction: Natural products with pharmacological activity require preclinical evaluations to justify their uses scientifically. The pyrogen assay is a safety pharmacology test performed to determine the presence of endotoxins and it is a valuable method to demonstrate the bio-derivative products safety and their prebiotic potential in the field of immunonutrition.
Objective: To evaluate the pyrogenicity of fungal bioproducts from Pleurotus ostreatus (aqueous extracts from mycelium and fruiting bodies) and a biopreparation from Kluyveromyces marxianus yeast, using a pyrogen assay in New Zealand rabbits.
Method: Concentrations of 1.0 and 10.0 mg/mL of each sample were tested intravenously at doses of 0.5 and 5.0 mg/kg body weight. The experimental design complied with good laboratory practices as established by the International Council for Laboratory Animal Science and was carried out according to the standard work procedures of the Centro de Toxicología y Biomedicina, Santiago de Cuba.
Results: Pleurotus ostreatus extracts and the yeast biopreparation (0.5 mg/kg) showed no signs of pyrogenicity. In the biopreparation results (5.0 mg/kg), temperature values fall in the uncertainty range according to the United States Pharmacopoeia (USP), and therefore it was suggested to repeat the study.
Conclusions: Pleurotus ostreatus extracts and Kluyveromyces marxianus biopreparation (0.5 mg/kg) did not induce a significant temperature increase in the animals, thereby suggesting that there are no endotoxin levels in such bioproducts that could cause pyrogenicity.


REFERENCES

  1. Llauradó G, Morris HJ, Heykers A, Lanckacker E, Cappoen D, Delputte P, et al. Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract from Pleurotus ostreatus Mycelium. J Fungi [Internet]. 2021 [citado 13/12/2021]; 7:206. DOI: https://doi.org/10.3390/jof70302061.

  2. Neun BW, Cedrone E, Potter TM, Crist RM, Dobrovolskaia MA. Detection of Beta-Glucan Contamination in Nanotechnology-Based Formulations. Molecules [Internet]. 2020 [citado 13/12/2021]; 25:3367. DOI: https://doi.org/10.3390/molecules.251533672.

  3. Morris HJ, Llauradó G, Beltrán Y, Lebeque Y, Batista PL, Moukha S, et al. Research work on the immunomodulating and antitumor properties of Pleurotus sp. in Cuba. En: Sánchez JE, Mata G, Royse DJ. Updates on Tropical Mushrooms. Basic and Applied Research. 1ed. [Internet]. San Cristóbal de Las Casas, Chiapas, México: El Colegio de la Frontera Sur; 2018. p. 159-180. [citado 13/12/2021]. Disponible en: https://biblioteca.ecosur.mx/cgi-bin/koha/opac-detail.pl?biblionumber=000053981

  4. Fortin O, Aguilar B, Dang K, Salmieri S, Lacroix M. Cancer Chemopreventive, Antiproliferative, and Superoxide Anion Scavenging Properties of Kluyveromyces marxianus and Saccharomyces cerevisiae var. boulardii Cell Wall Components. Nutrition and Cancer [Internet]. 2018 [citado 13/02/2022]; 70(1):83-96. DOI: https://dx.doi.org/10.1080/01635581.2018.13802044.

  5. Patel S, Goyal A. The current trends and future perspectives of prebiotics research: a review. Biotech [Internet]. 2012 [citado 13/02/2022]; 2:115-125. DOI: https://doi.org/10.1007/s13205-012-0044-x5.

  6. Prajitha N, Athira SS, Mohanan PV. Pyrogens, a polypeptide produces fever by metabolic changes in hypothalamus: mechanisms and detections. Immunol Letters [Internet]. 2018 Dec. [citado 13/02/2022]; 204:38-46. DOI: https://doi.org/10.1016/j.imlet.2018.10.0066.

  7. Franco E, Garcia V, Jiménez P, Garrosa M, Girbés T, Cordoba M, et al. Endotoxins from a pharmacopoeial point of view. Toxins [Internet]. 2018 [citado 13/02/2022]; 10(8):331. DOI: https://doi.org/10.3390/toxins100803317.

  8. Trudy MW, Kurt Z. Lipopolysaccharides in Food, Food Supplements, and Probiotics: Should We be Worried? Eur J Microbiol Immunol [Internet]. 2018 [citado 13/04/2022]; 8(3):63-69. DOI: https://doi.org/10.1556/1886.2018.000178.

  9. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem [Internet]. 1956 [citado 13/02/2022]; 28:350-356. DOI: https://doi.org/10.1021/ac60111a0179.

  10. Ferrer JC, Mas SM, Beltrán Y, Rodríguez Y, Morris HJ. Optimization of medium composition for the production of Pleurotus ostreatus biomassand phenolsin submerged fermentation with response. Tecn Quím [Internet]. 2019 [citado 13/02/2022]; 39(1):2224-6185. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S2224-6185201900010000110.

  11. Beltrán Y, Morris H, Domínguez O, Batista P, Llauradó G. Composición micoquímica y actividad antioxidante de la seta Pleurotos ostreatus en diferentes estados de crecimiento. Acta Biol Colomb [Internet]. 2021 [citado 13/02/2022]; 26(1):89-98. DOI: http://dx.doi.org/10.15446/abc.v26n1.8451911.

  12. Morris HJ, Llauradó G, Bermúdez RC, Cos P, Lebeque Y, Ramirez F, et al. Evaluation of the immunomodulatory activity of bioproducts obtained from the edible-medicinal mushroom Pleurotus ostreatus. Biotecnol Apl [Internet]. 2018 [citado 13/02/2022]; 35(3):3511-14. Disponible en: https://www.medigraphic.com/cgi-bin/new/resumenI.cgi?IDARTICULO=9562912.

  13. Babu MM, Rao AL, Harshitha V, Madhavi V, Lavanya VNS, Vineetha V, et al. Evaluation of Antipyretic Activity of Ethanolic Extract of Wedelia Trilobata. Int J Res [Internet]. 2018 [citado 13/02/2022]; 2(2):212-216. Disponible en: https://ijraps.in/index.php/ijraps13.

  14. Fernandes V, Silva DJ da, Alana I, Santos A, Paiva F de, Fernandes I, et al. A Comparison of Pyrogen Detection Tests in the Quality Control of Meningococcal Conjugate Vaccines: The Applicability of the Monocyte Activation Test. ATLA [Internet]. 2018 [citado 13/02/2022]; 46(5):255-272. DOI: https://doi.org/10.1177/02611929180460050614.

  15. Ahmad S, Abbasi WM, Rehman T. Evaluation of antipyretic activity of Belladonna and Pyrogenium ultrahigh dilutions in induced fever model. J Complement Integr Med [Internet]. 2019 [citado 13/02/2022]; 16(1). DOI: https://doi.org/10.1515/jcim-2018-012715.

  16. Zhang X, Wang Y, Li S, Dai Y, Li X, Wang Q, et al. The potential antipyretic mechanism of Gardeniae Fructus and its heat-processed products with plasma metabolomics using rats with yeast-induced fever. Front Pharmacol [Internet]. 2019 [citado 13/02/2022]; 10:491. DOI: https://doi.org/10.3389/fphar.2019.0049116.




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