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2022, Number 3

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Correo Científico Médico 2022; 26 (3)

Usefulness of PCR in the detection of Mycobacterium tuberculosis in clinical samples

Iglesias OS, Ruiz TC, Becerra AG

Language: Spanish
References: 25
Page:
PDF size: 273.14 Kb.


ABSTRACT

Introduction: Tuberculosis is a highly prevalent infectious disease worldwide.
Objective: To determine the diagnostic usefulness of M. tuberculosis by polymerase chain reaction technique (PCR) in patients from a hospital at northern Peru.
Methods: Clinical samples from patients were processed for DNA extraction by the salting out method. To determine if the pathogen in the sputum sample belongs to the M. tuberculosis complex, markers that amplify IS6110 were used, amplified using the PCR technique and then the PCR products were subjected to 1.5% agarose gel electrophoresis and visualized by staining with 1% ethidium bromide. The study was conducted at the Hospital Regional Lambayeque, Chiclayo, Lambayeque, Peru.
Results: A total of 148 patient samples were evaluated: 72 (48%) males and 77 (52%) females. Ages ranged from five to ninety-three years old, with an average age of 45.85 years old. It was observed that 105 (70%) patients tested negative for tuberculosis and 44 (30%) tested positive for tuberculosis. In addition, 24 (55%) positive male patients were detected as well as 20 (45%) negative female patients. In the samples that tested positive for TB, two abscess biopsy (5%), two intestinal biopsy (5%), seventeen bronchoalveolar lavage (39%), four cerebrospinal fluid (9%), seventeen pleural fluid (39%) and two peritoneal fluid (5%) were found.
Conclusions: Mycobacterium tuberculosis can be detected in pulmonary and extrapulmonary samples in hospital patients.


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