Gómez-Haro J, Vallejo-Cardona AA, Flores-Valdez MA

Language: Spanish
References: 15
Page: 19-24
PDF size: 456.36 Kb.

ABSTRACT

Introduction: biofilms are complex communities of microorganisms that adhere to each other and form an extracellular matrix that provides protection against antibiotics and the host's immune system, contributing to the chronicity and treatment resistance of diseases, such as tuberculosis. Similar structures to in vitro biofilms have been found in the lungs of animal models infected with Mycobacterium tuberculosis, which could increase the difficulty of treatment and the risk of relapse. This study explores the production of cellulose by Mycobacterium bovis BCG, including a recombinant strain that overexpresses the cellulase encoding gene celA1, and analyze them by Raman spectroscopy. The importance of understanding the interaction between cellulose and mycobacteria in developing therapeutic strategies and biotechnological applications is highlighted. Objective: to identify the presence of cellulose using Raman spectroscopy. Material and methods: cultures of Mycobacterium bovis BCG Pasteur ATCC 35734 strain, as well as a recombinant strain that overexpresses the cellulase-encoding gene celA1, were carried out in Sauton medium without detergent, to promote autoaggregation and biofilm formation; material was extracted using a bacterial cellulose protocol and Raman spectra were compared to those of commercial cellulose. Results: although differences are observed in the resolution and location of some peaks, those characteristic of cellulose confirm its presence in the strains under study. Conclusion: through Raman spectra analysis, we found that Mycobacterium bovis BCG produces cellulose in cultures in Sauton medium without detergent, since its spectra show similarities to those of commercial microcrystalline cellulose.

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