Ges NAA, Viltres CH, García HL, Ponce RL, Aguilera CY, Luz I, Botelho K, Fontes W

Language: English
References: 37
Page:
PDF size: 696.32 Kb.

ABSTRACT

Nowadays, there is a growing interest in biodegradable polymers-based materials due to their diverse application in the biomedical field. Most studied systems involve biocompatible micro and nanodevices, such as liposomes, dendrimer, micelles or polymeric nanogels. The use of Radiation Technology, specifically gamma radiation, to produce micro and nanogels raises the possibility to obtain higher purity products, an important feature for biomedical and pharmaceutical applications. The radio-induced synthesis, characterization, cytotoxicity evaluation, and immunological response of nanogels are described in this study. Nanogel synthesis was performed in the absence of oxygen using aqueous polyvinylpyrrolidone solutions. Crosslinking reactions were carried out at 25 °C in a gamma irradiation chamber with a 60Co source. Nanogels properties were analysed by Scanning Electron Microscopy, Attenuated Total Reflection-Fourier Transform Spectroscopy, Dynamic Light Scattering, and Viscosimetry. The cytotoxicity and immunological response were evaluated by MTT test and analysis of the neutrophil respiratory burst. The results showed that nanogels formation strongly depends on the total absorbed dose. The nanogels have an elliptical shape and their chemical structure is similar to the initial polymer. The nanogels are biocompatible and promote a low-intensity neutrophil activation, similar to the well-characterized biomaterial TiO2, suggesting their potential biomedical uses.

REFERENCES

1. Vauthier C, Ponchel G. Polymer Nanoparticles for Nanomedicines A guide for the design, Preparation and Development. France: Springer; 2016. p. 635.

2. Sultana F, Manirujjaman M, Imran-Ul-Haque M, Arafat M, Sharmin S. An overview of nanogel drug delivery system. J Appl Pharm Sci. 2013;3(8 Suppl):95-105.

3. Fakhari A, Subramony JA. Engineered in-situ depot-forming hydrogels for intratumoral drug delivery. J Controlled Release. 2015;220:465-75.

4. Amini-Fazl MS, Mohammadi R, Kheiri K. 5Fluorouracil loaded chitosan/polyacrylic acid/Fe3O4 magnetic nanocomposite hydrogel as a potential anticancer drug delivery system. Int J Biol Macromol. 2019;132:506-13.

5. Pan Q, Lv Y, Williams GR, Tao L, Yang H, Li H. Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems. Carbohydr Polym. 2016;151:812-20.

6. Mudassir A, Muhammad S, Muhammad UM, Shahzeb K, Zahid H, Arshad M, et al. Novel biodegradable pH-sensitive hydrogels: An efficient controlled release system to manage ulcerative colitis. Int J Biol Macromol. 2019;136:83-96.

7. Rigogliuso S, Sabatino MA, Adamo G, Grimaldi N, Ghersi G, Dispenza C. Polymeric Nanogels; Nanocarriers for Drug Delivery Application. Chemical Engineering Transactions. 2012;27:247-52.

8. Bolla PK, Rodriguez VA, Kalhapure RS, Kolli CS, Andrews S, Renukuntla J. A review on pH and temperature responsive gels and other less explored drug delivery systems. J Drug Deliv Sci Technol. 2018;46:416-35.

9. Kabanov AV, Vinogradov SV. Nanogels as Pharmaceutical Carriers. 2008:67-80.

10. Akhtar MF, Hanif M, Ranjha NM. Methods of synthesis of hydrogels. A review. Saudi Pharm J. 2016;24(5):554-9.

11. Kadlubowski S. Radiation-induced synthesis of nanogels based on poly(N-vinyl-2-pyrrolidone). A review. Radiat Phys Chem. 2014;102:29-39.

12. Cruz A, Ortega A, García-Uriostegui L, Isoshima T, Burillo G. Radiation grafting of N-vinylcaprolactam onto nano and macrogels of chitosan: Synthesis and characterization. Carbohydr Polym. 2017;155:303-12.

13. Leyva-Gómez G, Santillan-Reyes E, Lima E, et al. A novel hydrogel of poloxamer 407 and chitosan obtained by gamma irradiation exhibits physicochemical properties for wound management. Mater Sci Eng C. 2017;74:36-46.

14. Bayramgil NP. Synthesis, characterization and drug release behavior of poly (1-vinyl 1,2,4-triazole) hydrogels prepared by gamma irradiation. Colloids Surf B. 2012;97:182-9.

15. Ges AA, Naranjo G, Viltres Cobas H, Fonseca Rodriguez D, Rapado Paneque M, Aguilera Corrales Y. Radiation-Induced Synthesis of Polyvinylpyrrolidone (PVP) Nanogels. J Phys Sci Appl. 2016;6(5).

16. NC. Metrological Assurance. Capillary viscometers. General rules for viscosity measurements. 1981.

17. ISO. Plastics-Determination of the viscosity of polymers in dilute solution using capillary viscometer. 1998. Report No. 1628-1.

18. Rosiak JM. Nano- and microgels of poly (vinyl methyl ether) obtained by radiation techniques. Eurasian Chemico-Technological Journal. 2007;9:165-76.

19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63.

20. Teles L, Aquino E, Neves A, Garcia C, Roepstorff P, Fontes B, et al. Comparison of the Neutrophil Proteome in Trauma Patients and Normal Controls. Protein Pept Lett. 2012;19(6):663-72.

21. Lindemann O, Strodthoff C, Horstmann M, Nielsen N, Jung F, Schimmelpfennig S, et al. TRPC1 regulates fMLP-stimulated migration and chemotaxis of neutrophil granulocytes. Biochim Biophys Acta Mol Cell Res. 2014;1853(9):2122-30.

22. Ges AA, Viltres H, Borja R, Rapado M, Aguilera Y. Gamma radiation-induced synthesis and characterization of Polyvinylpyrrolidone nanogels. En: Journal of Physics: Conference Series. IOP Publishing; 2017. p. 012080.

23. Baehner RL, Nathan DG. Quantitative nitroblue tetrazolium test in chronic granulomatous disease. N Engl J Med. 1968;278(18):971-6.

24. Hsu KH, Chauhan A. Rapid and selective removal of preservative from ophthalmic formulations during eyedrops instillation. Eur J Pharm Biopharm. 2015;97:30-8.

25. Soni G, Yadav KS. Nanogels as potential nanomedicine carrier for treatment of cancer: A mini review of the state of the art. Saudi Pharm J. 2016;24(2):133-9.

26. Marek SR, Conn CA, Peppas NA. Cationic nanogels based on diethylaminoethyl methacrylate. Polymer. 2010;51(6):1237-43.

27. Ding Y, Shen SZ, Sun H, Sun K, Liu F, Qi Y, et al. Design and construction of polymerized-chitosan coated Fe3O4 magnetic nanoparticles and its application for hydrophobic drug delivery. Mater Sci Eng C. 2015;48:487-98.

28. Ma Z, Ma R, Wang X, Gao J, Zheng Y, Sun Z. Enzyme and PH responsive 5-flurouracil (5- FU) loaded hydrogels based on olsalazine derivatives for colon-specific drug delivery. Eur Polym J. 2019;118:64-70.

29. Ulanski P, Janik I, Rosiak JM. Radiation formation of polymeric nanogels. Radiat Phys Chem. 1998;52(1-6):289-94.

30. Ulanski P, Rosiak JM. The use of radiation technique in the synthesis of polymeric nanogels. Nucl Instrum Methods B. 1999;151:356-60.

31. AbdEl-Rehim H, Hegazy EA, Eid A, Amr AA. Radiation Induced Polyvinylpyrrolidone / Polyacrylic Acid Nano-Gel Formation for Biomedical Applications. Nanosci Nanotechnol. 2010:71-87.

32. Duygu Sütekin S, Güven O. Application of radiation for the synthesis of poly(n-vinylpyrrolidone) nanogels with controlled sizes from aqueous solutions. Appl Radiat and Isotop. 2019;145:161-9.

33. Ghaffarlou M, Sütekin SD, Güven O. Preparation of nanogels by radiation-induced cross-linking of interpolymer complexes of poly (acrylic acid) with poly (vinyl pyrrolidone) in aqueous medium. Radiat Phys Chem. 2018;142(April):130-6.

34. Vecchione R, Quagliariello V, Giustetto P, Calabria D, Sathya A, Marotta R, et al. Oil/water nano-emulsion loaded with cobalt ferrite oxide nanocubes for photo-acoustic and magnetic resonance dual imaging in cancer: in vitro and preclinical studies. Nanomedicine Nanotechnol Biol Med. 2017;13(1):275-86.

35. Manford K, Patterson J. Measurement of Growth and Viability of Cells in Culture. 1979;LVIII(1974).

36. García-Rodríguez LM, Díaz-Jiménez D, Pérez-Guevara OL, Ges Naranjo A. Evaluación de la citotoxicidad de nanogeles de polivinilpirrolidona (PVP) en fibroblastos murinos. Revista Biomédica. 2019;30:131-6.

37. Zysk SP, Gebhard H, Plitz W, Buchhorn GH, Sprecher CM, Jansson V, et al. Influence of orthopedic particulate biomaterials on inflammation and synovial microcirculation in the murine knee joint. J Biomed Mater Res - Part B Appl Biomater. 2004;71(1):108-15.