2021, Number S1
Antibacterial and Antifungal Activity of the essential oil of Minthostachys mollis against oral microorganisms
Language: Spanish
References: 20
Page:
PDF size: 625.04 Kb.
ABSTRACT
Introduction: Minthostachys mollis is an aromatic plant species growing in Latin America which produces essential oils with antimicrobial activity.Objective: Determine the activity of essential oil from Minthostachys mollis at various concentrations as compared with doxycycline and fluconazole against Porphyromonas gingivalis, Staphylococcus aureus and Candida albicans at 24, 48 and 72 hours.
Methods: An in vitro experimental longitudinal study was conducted. Fifteen wells were prepared per subgroup to evaluate the inhibitory effect of all concentrations, for a sum total of 360 wells. Chemical components of the essential oil were identified by gas chromatography-mass spectrometry. The inhibitory effect was analyzed with the Kirby-Bauer diffusion method in Mueller-Hinton and Columbia agar. Statistical analysis was based on ANOVA and Tukey's test.
Results: Chemical analysis mainly found pulegone (30.17%) and menthone (16.55%). The inhibition halos of 100% Minthostachys mollis at 24, 48 and 72 hours against Porphyromonas gingivalis measured 10.2 mm, 9.8 mm and 9.6 mm, respectively, against Staphylococcus aureus they measured 10.4 mm, 9.7 mm and 9.4 mm, respectively, and against Candida albicans they measured 9.8 mm, 8.9 mm and 8.5 mm, respectively. The antimicrobial effect of Minthostachys mollis at all concentrations was significantly lower than that of fluconazole and doxycycline (p < 0.001).
Conclusions: The essential oil from 100% Minthostachys mollis displayed its best inhibitory activity against Staphylococcus aureus, Porphyromonas gingivalis and Candida albicans at 24 hours. However, such antimicrobial effect decreases with the passing of time.
REFERENCES
Melo-Guerrero MM, Ortíz-Jurado DE, Hurtado-Benavides AM. Comparación de la composición y de la actividad antioxidante del aceite esencial de manzanilla (Matricaria chamomilla L.) obtenido mediante extracción con fluidos supercríticos y otras técnicas verdes. Rev Acad Colomb Cienc Ex Fis Nat. 2020 [acceso 01/12/2020];44(172):845-56. Disponible en: https://raccefyn.co/index.php/raccefyn/article/view/8627.
Rojas-Armas JP, Arroyo-Acevedo JL, Ortiz-Sánchez JM, Palomino-Pacheco M, Hilario-Vargas HJ, Herrera-Calderón O, et al. Potential Toxicity of the Essential Oil from Minthostachys mollis: A Medicinal Plant Commonly Used in the Traditional Andean Medicine in Peru. J Toxicol. 2019;2019:1987935. DOI: http://dx.doi.org/10.1155/2019/1987935
Torrenegra M, Granados C, Durán M, León G, Yáñez X, Martínez C, et al. Composición Química y Actividad Antibacteriana del Aceite Esencial de Minthostachys mollis. Orinoquia. 2016 [acceso 17/11/2020];20(1):69-74. Disponible en: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-37092016000100008&lng=en10.
Campo M, Ambuludí D, Cepeda N, Márquez I, San Martín D, Cuesta O. Composición química y actividad antibacteriana del aceite esencial de Minthostachys mollis Griseb contra el Staphylococcus aureus. Rev Cuba Farm. 2017 [acceso 22/10/2020];51(2). Disponible en: http://revfarmacia,sld,cu/index,php/far/article/view/183/17512.
Rojas J, Ruiz JQ, Almonacid R, Ortiz J, Palomino M, Huaroto L, et al. Antibacterial Activities of Essential Oils from Three Medicinal Plants in Combination with EDTA against Methicillin-resistant Staphylococcus aureus. Microbiol Res J Int. 2017;17(4):1-10. DOI: https://doi.org/10.9734/BMRJ/2016/29666
Alcala K, Alvarado G, Alejandro A, Huayane E. Actividad Antimicótica del aceite esencial de las hojas de Minthostachys mollis (Muña) comparado con el Fluconazol en cultivo de Candida albicans. CIMEL. 2011 [acceso 22/10/2020];16(2):83-6. Disponible en: https://www.cimel.felsocem.net/index.php/CIMEL/article/view/204/15314.
Huari G. Efecto antibacteriano in vitro del aceite esencial de Minthostachys mollis (muña) en Streptococcus mutans. [Tesis de licenciatura]. Lima: Universidad Nacional Mayor de San Marcos; 2014. [acceso 24/10/2020]. Disponible en: Disponible en: https://cybertesis.unmsm.edu.pe/bitstream/handle/20.500.12672/3680/Huari_gg.pdf?sequence=1&isAllowed=y 15.
Fuertes C, Munguía Y. Estudio comparativo del aceite esencial de Minthostachys mollis (Kunth) Griseb (muña) de tres regiones peruanas por cromatografía de gases y espectrometría de masas. Ciencia e Investigación. 2001 [acceso 03/11/2020];4(1):23-39. Disponible en: https://revistasinvestigacion,unmsm,edu,pe/index,php/farma/article/view/338916.
Benites J, Guerrero A, Salas F, Martínez J, Jara R, Venegas E, et al. Chemical composition, in vitro cytotoxic and antioxidant activities of the essential oil of Peruvian Minthostachys mollis Griseb. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 2018 [acceso 25/11/2020];17(6):566-74. Disponible en: https://pesquisa.bvsalud.org/portal/resource/pt/biblio-100733618.
GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017 [acceso 02/11/2020];390(10100):1211-59. Disponible en: https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(17)32154-2/fulltext19.