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2007, Number 2

Bioquimia 2007; 32 (2)

A modified method for cloning adherent mammalian cells

Maldonado V, Meléndez-Zajgla J
Full text How to cite this article

Language: English
References: 6
Page: 70-72
PDF size: 76.50 Kb.


Key words:

Cell culture, dilution cloning, mammalian cells.

ABSTRACT

Dilution cloning of adherent mammalian cells is a very frequent practice in most molecular and cell biology laboratories. It is commonly used for isolation of specific biochemical mutants, and cell strains with marker chromosomes. In this report, we present a modified and simple dilution cloning method that allows even inexperienced workers to successfully clone adherent mammalian cells.


REFERENCES

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  3. Drexler HG, Dirks WG, MasLeod RA. False human hematopoietic cell lines: cross-contamination and misinterpretation. Leukemia. 1999; 13: 1601-1607.

  4. Puck TT, Marcus PI. A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors. Proc Natl Acad Sci. (USA) 1955; 41: 432-437.

  5. Macpherson I. Soft agar techniques. In: Kruse PF, Patterson JK. (Eds). Tissue culture methods and applications. New York: New York Academy Press; 1992. p. 276-280.

  6. Buick RN, Stanisic TH, Fry SE, Salmon SE, Trent JM, Krosovitch P. Development of an agar-methylcellulose clonogenicity assay for cells of transitional cell carcinoma of the human bladder. Cancer Res. 1979; 39: 5051-5056.




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CÓMO CITAR (Vancouver)

Bioquimia. 2007;32