Quintana AB, Rodríguez JV, Guibert EE

Language: English
References: 0
Page: 270-271
PDF size: 120.65 Kb.

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Rat livers were cold stored (0°C-48 Hs) in University of Wisconsin (UW) solution. To reduce cold preservation/reperfusion injuries, three concentrations (50, 100 and 250 µM) of S-nitrosoglutathione (GSNO) were studied. GSNO, which releases Nitric Oxide (NO), was added into UW solution during the cold storage period. NO is a vasodilator that acts on hepatic microvascular system protecting the liver from preservation/reperfusion injuries. We compared five groups of livers: a) Normal control (livers neither stored not reperfused); b) cold stored livers for 48 Hs in UW; c) cold stored livers for 48 Hs in UW + 50 µM GSNO; d) cold stored livers for 48 Hs in UW + 100 µM GSNO; e) cold stored livers for 48 Hs in UW + 250 µM GSNO. Groups b, c and e were reperfused after cold storage. Pieces of rat livers were histological processed for paraffin embedded after reperfusion. Liver slices were stained with Direct Red 80 – Fast Green and analyzed with polarized microscopy to differentiate collagen type I from type III.