medigraphic.com
ISSN 0188-2198 (Print)

2007, Number 4

<< Back Next >>

Rev Mex Cardiol 2007; 18 (4)

Aging, adrenergic vascular reactivity and the synthesis of nitric oxide in rat aortic rings

Pasos-Peniche JE, Miranda-Díaz AG, Totsuka-Sutto S, Hernández-Ojeda J, Pascoe-González S, Garibay C, Cardona-Muñoz EG

Language: Spanish
References: 22
Page: 173-180
PDF size: 147.55 Kb.


ABSTRACT

Background: It is known that aging has an impact on endothelial function and on nitroergic and adrenergic responses; however, it is not known how these interactions are modified with age. Aim: To evaluate the effect of aging on the adrenergic vascular response and its relationship with endothelial Nitric Oxide synthesis.
Material and methods: Using Furchgott’s method isolated rat aortic cylinders of 10-12, 20-22 and greater than 40 weeks of age. The response (contraction/relaxation) to adrenalin, acetylcholine and potassium nitrite was evaluated in the presence or absence of functional endothelium and in the presence or absence of L-Arginine as a nitric oxide synthesis facilitator. Dose-effect cumulative curves were drawn. Results: There was an inverse relationship between age and the endothelial ability to produce nitric oxide and its vascular dilatory response to acetylcholine; age also increased the contractile response to noradrenalin and didn’t modify the effects of potassium nitrite. The presence of endothelium favored vasodilatory response while its absence, mechanic or functional, increased the contractile response. Conclusions: Aging reduces endothelial nitric oxide synthesis, increases the contractile response to noradrenalin, diminishes the vasodilatory response to acetylcholine and doesn’t affect the response to Potassium Nitrite.


REFERENCES

  1. Gerhard M, Roddy MA, Creager SJ, Creager MA. Aging progressively impairs endothelium-dependent vasodilation in forearm resistance vessels of humans. Hypertension 1996; 27: 4.

  2. Murad F, Arnold WP, Mittal CK, Braughler JM. Properties and regulation of guanylate cyclasa and some proposed functions for cyclic GMP. Adv Cyclic Nucleotide Res 1979; 11: 175-204.

  3. Rubanyi GM, Vanhoutte PM. Superoxide anions and hyperoxia inactivate endothelium-derived relaxing factor. Am J Physiol 1986; 250: 822-827.

  4. Getz GS, Reardon CA. Arginine/Arginase NO NO NO. Arterioscler Thromb Vasc Biol 2006; 26(2): 237-239.

  5. Moncada S, Higgs A. The L-arginine-nitric oxide pathway. N Engl J Med 1993; 329: 2-12.

  6. Furchgott RF. The 1996 Albert Lasker Medical Research Awards. The discovery of endothelium-derived relaxing factor and its importance in the identification of nitric oxide N. JAMA 1996; 276: 186-188.

  7. Umans JG, Levi R. Nitric oxide in the regulation of blood flow and arterial Pressure. Annu Rev Physiol 1995; 57: 771-90.

  8. Furchgott RF. Vasodilatation: vascular smooth muscle, peptides, and endothelium. NY: Raven Pres 1988; 401-418.

  9. Bataineh A, Raij L. Angiotensin II, nitric oxide and end-organ damage in hypertension. Kidney Int 1998; 68: S14-S19.

  10. Miyagawa K, Ohashi M, Yamashita S, Kojima M, Sato K, Ueda R et al. Increased oxidative stress impairs endothelial modulation of contractions in arteries from spontaneously hypertensive rats. J Hypertension 2007; 25(2): 415–421.

  11. Taddei S, Virdis A, Mattei P, Ghiadoni L, Fasolo CB, Sudano I et al. Hypertension causes premature aging of endothelial function in humans. Hypertension 1997; 29:736-743.

  12. Furchgott RF, Bhadrakom S. Reactions of strips of rabbit aorta to epinephrine, isopropyl-aterenol, sodium nitrite and other drugs. J Pharmacol ExpTher 1953; 108: 129-143.

  13. Ahlquist RP. Development of the concept of alpha and beta adrenotropic receptors. Ann NY Acad Sci 1966; 549-552.

  14. Gray SD. Effect of angiotensin II on neonatal lamb carotide arteries. Experientia 1976; 32: 350-351.

  15. Ferri C, Grassi D, Grassi G. Cocoa beans, endothelial function and aging: an unexpected friendship? J Hypertension 2006; 24(8): 1471–1474.

  16. John S, Schmieder RE. Impaired endothelial function in arterial hypertension and hypercholesterolemia: potential mechanisms and differences. J Hypertension 2000; 18: 363-374.

  17. Chauhan A, More RS, Mullins PA, Taylor G, Petch C, Schofield PM. Aging-associated endothelial dysfunction in humans is reversed by L-arginine. J Am Coll Cardiol 1996; 28: 1796-1804.

  18. Imaizumi T, Hirooka Y, Masaki H, Harada S, Momohara M, Tagawa T, Takeshita A. Effects of L-arginine on forearm vessels and responses to acetylcholine. Hypertension 1992; 20: 511-517.

  19. Heiss C, Schroeter H, Balzer J, Kleinbongard P, Matern S, Sies H et. al. Endothelial function, nitric oxide, and cocoa flavanols. J Cardiovasc Pharm 2006; 47(2): S128-S135.

  20. Panza JA, Casino PR, Kilcoyne CM, Quyyumi AA. Role of endothelium-deriven nitric oxide in the abnormal endothelium –dependent vascular relaxation of patients with essential hypertension. Circulation 1993; 87: 1468-1474.

  21. Taddei S, Virdis A, Ghiadoni L, Magagna A, Salvetti A. Ciclooxigenase inhibition restores nitric oxide activity in essential hypertension. Hypertension 1997; 29: 274-279.

  22. Stroes ES, Koomans HA, de Bruin TW, Rabelink TJ. Vascular function in forearm of hypercholesterolemic patients of and on lipid-lowering medication. Lancet 1995; 346: 467-471.




2020     |     www.medigraphic.com