Hernández GCA, Frigolet VVME, Herrerías CT, Flores HH, Meraz CN

Language: Spanish
References: 11
Page: 3-12
PDF size: 121.70 Kb.

ABSTRACT

Objective: To evaluate the hydrogen peroxide ability to induce the synthesis of matrix metalloproteinase-9 (MMP-9) in an in vitro chorioamniotic membrane model. Material and methods: An experimental, transversal, analytic and prospective study was made. From amniotic membranes (n = 13) of term labour pregnant women chorionic and chorioamniotic explants were prepared; these were stimulated with hydrogen peroxide [200 mcM]. A group of explants were cultured with vitamin C, 48 hours prior to the hydrogen peroxide stimulus. Twenty-four hours after hydrogen peroxide stimulus the supernatants were collected and metalloproteinase-9 production was determined by zymography methods.
Results: Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C prior to the hydrogen peroxide stimulus, the MMP-9 production diminished (p ‹ 0.05; Dunn’s method).
Conclusions: Hydrogen peroxide induced the synthesis of metalloproteinase-9 in chorionic and chorioamniotic membranes. When chorionic and chorioamniotic membranes were cultured with vitamin C, 48 hours prior to peroxide hydrogen stimulus, the metalloproteinase-9 production diminished with statistical significance.

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