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ISSN 0185-6014 (Print)
Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2008, Number 1

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Rev Mex Patol Clin Med Lab 2008; 55 (1)

Determination of sex by means of molecular analysis of the amelogenin gene

Velarde-Félix JS, Molina-Benítez CE, Solórzano-Rosales SR, Cázarez-Salazar SG, Rendón-Aguilar H, Murillo-Llanes J, Ríos-Tostado JJ

Language: Spanish
References: 9
Page: 17-20
PDF size: 95.66 Kb.


ABSTRACT

Amelogenin test is often use as a forensic analyses for sex typing. Objective: Based in the amelogenin gene test, to demonstrate the efficacy of a non commercial molecular system for sex identification. Material and methods. A blind transversal, observational and comparative study. DNA was obtained from 457 blood donors wish to participate providing five milliliters of peripheral blood; these samples where amplified by Polymerase Chain Reaction of a region of the amelogenin gene, located in both sexual chromosomes («AMELX» for gene of X chromosome and «AMELY» for gene of Y chromosome). Results: 239 women and 218 men were screened; comparing our results with the participant’s sex and names. Using Epilnfo V6 statistical program, we obtained a 100% of concordance, and 100% of sensibility, specificity and positive and negative predictive values, as well as kappa coefficient of 1 (p › 0.05). Conclusions: We conclude this test is highly reliable to this Mexican population for sex typing.


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