Lucena SR, Ginel PPJ, López RR, Novales DM, Martín SE, Molleda CJM

Language: English/Spanish
References: 9
Page: 191-195
PDF size: 420.21 Kb.

ABSTRACT

Serum TLI (Trypsin-like-immunoreactivity) determination for the diagnosis of exocrine pancreatic insufficiency in the cat remains as an experimental technique; thus, coprologic analyses are still required tO make a diagnosis of exocrine pancreatic insufficiency in this species. For this purpose, concentrations of steatrrea, amylorrea, creatorrea and of fecal amylase and trypsine in normal cats are required. Three fecal samples obtained during three consecutive days from 36 healthy cats were studied. Steatorrea, amylorrea and creatorrea levels were determined by microscopic analysis after Sudan III and lugol's iodine staining, respectively. Fecal amylase and trypsine were detected by radial enzyme diffusion. Results of every parameter were highly variable (0-187 fat droplets/10 fields and 0-23 muscle fibers/10 field. Starch granules were not found in most of the cats; fecal amilase ranged from 4 to 17 mm and fecal tripsine from 4 to 18 mm), and all of these parameters should be considered to confirm a diagnosis. Fecal amylase and trypsine values lower than 7 mm with higher fat, starch and muscle fibers levels are indicative of exocrine pancreatic insufficiency. Doubtful results would require a new fecal analysis after a three day diet with high content of fat, proteins and starch to assess pancreatic function.

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