1997, Number 1
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Vet Mex 1997; 28 (1)
The effect of passive immunization on the Shwartzman reaction in the lung of rabbits induced by Pasteurella haemolytica lipopolysaccharide
Ramírez RR, Brogden KA, Cutlip RC
Language: English/Spanish
References: 21
Page: 13-20
PDF size: 1003.64 Kb.
ABSTRACT
The local Shwartzman reaction (SR) can be induced in the lungs of rabbits with
Pasteurella haemolytica (type Al) lipopolysaccharide (LPS). Lesions resemble, in part, those seen in pneumonic pasteurellosis of cattle. In this study, the effect of antibodies on the intensity of the SR was examined in rabbits. One group was passively immunized with 4 ml of antiserum obtained from rabbits immunized with a whole-cell bacterin (
P. haemolytica strain 82-25, IHA titre to strain 82-25 LPS was 1:2560). The other group was not immunized. At 24 h post-immunization (PI) rabbits in both groups were subdivided. The SR was induced in both, immunized and non-immunized rabbits by injecting a 50 Ng preparatory dose of LPS intratracheally (IT), followed 24 h later (48h PI) by a 100 Ng provocative dose of LPS intravenously (IV). Control groups received LPS IT/pyrogen free saline (PFS) IV or PFS IT/LPS IV. At 12 h after IV inoculation (60 h PI), all rabbits were euthanized and exsanguinated. Sections of the right lung were taken to determine histopathological changes and the left lung was lavaged (bronchoalveolar lavages [BAL]) to determine total and differential cell counts. The histopathological changes seen in the SR were similar to those seen in rabbits that received LPS IT/PFS IV, indicating that lesions were primarily in response to IT LPS inoculation. Passive immunization of rabbits resulted in a variable inflammatory response to the LPS; some rabbits had mild lung lesions, but others had more severe lesions than non-immunized rabbits. There were also differences in the total and differential BAL cell counts between immunized and non-immunized rabbits. The passive immunized groups had higher cell counts. It can be concluded that, IT LPS inoculation was more inflammatory than IV LPS and IT LPS/IV LPS (SR) in either passively or non-immunized rabbits and that passive immunization exacerbates the lung inflammatory response.
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