1997, Number 1
<< Back Next >>
Vet Mex 1997; 28 (1)
Oestrogenic syndrome in dairy cows by alfalfa comsuption with large amount of coumestrol
Romero-RCM, Tarragó CMR, Muñoz MR, Arista RR, Rosado GA
Language: English/Spanish
References: 23
Page: 25-30
PDF size: 466.33 Kb.
ABSTRACT
An oestrogenic syndrome caused in dairy cows after ingesting important concentrations of coumestrol is described. The syndrome is characterized by increased levels of repeat-breeding cows, abortions, metritis and ovarian cysts, as well as other estrogenic manifestations like: Increased cervical mucus (CM) fluidity and turgent uterus (TU) at the palpation with positive diagnosis of gestation between 40 and 60 days of pregnancy. This study was performed for one year in 608 cows in risk, in which 1264 inseminations achieved only 376 gestations. Of this, 102 cursed with TU and CM fluid; 36 ended in abortion and only 238 had normal pregnancies. Electrical resistance measurements in CM of pregnant cows (68.8 ± 15.17
vs. 114.03 ± 17.05 Ω, n = 30) and are similar to those observed in non-pregnant estrous animals (63.16 ± 10.56 Ω, n = 30). Radioimmunoanalysis determination of estradiol in 10 pregnant cows with TU and fluid CM proportioned normal values of plasma estradiol (12.9 ± 10.04 pg/ml) in 6/10 animals, levels which did not explain the presence of the estrogenic syndrome. Measurement of coumestrol concentration in the alfalfa ingested by the animals demonstrated the presence of important quantities of this compound (66.8 mg/kg dry alfalfa, without considering the losses during the extraction and purification procedures), which will undoubtedly explain the occurrence of the described syndrome. Economic and biological importance of the presence of this estrogenic pathology in Mexico is discussed.
REFERENCES
Adams, N. R.: Permanent infertility in ewes exposed to plant oestrogens. Aust. vet. J, 67: 197-201 (1990).
Bickoff, E.M., Livingston, A.L., Hendrickson, A.P. and Booth, A.M.: Relative potencies of several estrogen like compounds found in forages. Agric. Food Chem., 10: 410-412 (1962).
Coop, I.E. and Clark, V.R.: The reproductive performance of ewes mated on luzerne. N. Z. J. Agric. Res., 3: 922-933 (1960).
Dewick, P.M. and Martin, M.: Biosynthesis of pterocarpan, isoflavan and cooumestan metabolites of Medicago sativa: Chalcone, isoflavone and isoflavanone precursors. Phytochemistry, 18: 597-602(1979).
Engle, P.H., Bell, DJ. and Davis, P.R.: The effect of ladino clover, Bridsfoot Trifoil and Blue-grass pasture on the rate of conception among ewes. Can. J. Anim. Sci., 16: 703-710 (1957).
Friedman, P.: GB-Stat Version 3.0 Computer Aided Statistics & Graphics Tutorial/Manual. Howard University, Silver Spring, Maryland, 1991.
Feet, W.F. and Osman, S.F.: Inhibition of bacteria by the soybean isoflavonoids glyceollin and coumestrol. Phytopathology, 72: 755760(1982).
Herrera, D.M.R., Luna, M.M. y Romero, R.C.M.: Obtención de anticuerpos contra progesterona y estradiol, estandarización del radioinmunoanálisis y validación en suero de rumiantes. Vet. Méx., 24:223-230 (1993).
Holstein de México: Ganaderías con producciones de 7,500 a más de 11,000 kilos de leche. México-Holstein, 26(12):32 (1995).
Jurado, C.R.: Fitoestrógenos, plantas estrogénicas, hongos estrogénicos. En: Toxicología Veterinaria. Editado por: Jurado, C.R., 367-497. Salvat Editores, Barcelona, España, 1989.
Kelly, G.E., Joannou G.E., Reeder, A.Y., Nelson, C. and Waring, MA.: The variable metabolic response to dietary isoflavones in humans. Proc. Soc. exp. Biol. Med., 208:40-43 (1995).
Le Bars, J., Le Bars, P. et Brice, G.: Présence accumulation et devenir du coumestrol dans la luzerne et ses dérivés. Rec. Med. Vét., 166: 463-469 (1990).
Lookhart, G.L. and Finney, KF.: Determination of coumestrol in soybeans by high-performance liquid and thin-layer chromatography. Cereal Chem., 55: 967-972 (1978).
Lotane, E. and Adler, J.K.: Early effects of excessive alfalfa feeding on bovine fertility. Refuah. Vet., 23:102-110 (1966).
Neill, O.MJ., Adesanya, S.A., Roberts, M.F. and Pantry, I.R.: Inducible isoflavonoids from the lima bean, Phaseolus lunatus. Phytochemistry, 25:1315-1322 (1986).
Newsome, F.E. and Kitts, W.D.: Effects of alfalfa consumption on estrogen levels in ewes. Can J Anim. Sci., 57:531-534 (1977).
Newsome, F.E. and Kitts, W.D.: The effects of feeding coumestrol on the reproductive organs of prepuberal lamb. Can J. Anim. Sci., 60: 53-58 (1980).
Perel, E. and Under, H.R.: Dissociation of uterotrophic action from implantation-inducing activity in two non-steroidal oestrogens (coumestrol and genistein). J.Reprod. Fert., 21:171-175 (1970).
Romero-Ramírez, C.M, Landeta, M.L.A., Tarragó, C.M.R, Sánchez-Aldana, P.A., Casas, C.A. y Valencia, M.J.: Respuesta endocrina asociada a la eficiencia de tres tratamientos hormonales en vacas con quistes foliculares. Vet. Méx., 26:195-202 (1995).
Rossiter, R. and Beck, A.D.: Physiological and ecological studies on the oestrogenic isoflavones in subterranean clover. I. Effects of temperature. Austr. J. agric. Res., 17: 29-37 (1966).
Shemesh, M., Lindner, H.R. and Ayalon, N.: Affinity of rabbit uterine oestradiol receptor for phytooestrogens and its use in a competitive protein-binding radioassay for plasma coumestrol. J. Reprod. Fert., 29: 1-9 (1972).
Sukumaran, K. and Gnanamanickam, S.S.: Isolation of antifungal compounds form Indian fodder legume plants. Indian J. Microbial, 20: 204-207 (1980).
Valderrabano J.: Alteraciones reproductivas asociadas al consumo de fitoestrógenos. Invest. Agric. Prod. Sanid. Anim., 7: 115-124(1992).